黄芪多糖对气阴两虚Lewis肺癌荷瘤小鼠肿瘤生长、转移及细胞周期的影响
DOI: 10.3971/j.issn.1000-8578.2018.17.0220
Keywords: 黄芪多糖,气阴两虚,Lewis肺癌,细胞周期,抗瘤增效,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Effect of STAT1 on Cell Cycle of Human Glioma U251 Cells and Related Mechanism,Role of Gadd45 Protein Family in Tumorigenesis and Development,Effect of Cyclin G1 on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells and Its Mechanism,Effect of IL-4/IL-4R on Biological Behavior of Hepatocellular Carcinoma and Its Underlying Mechanism,Progress of Relationships of Calprotectin with Tumours,GM-CSF Enhances Effect of TMZ on High-grade Glioma Cells,Effects of Gefitinib Combined with Bruceolic Oil Emulsion on Human Lung Adenocarcinoma Cell Lines and Its Mechanism,Effects of SHP-1 Overexpression on Biological Characteristics of K562 Cells,Effect of Silencing Toll-like Receptor 4 Expression on Cell Cycle and Apoptosis of Human Lung Adenocarcinoma Cells A549,Tumor Suppressor Roles of miR-100 in Esophageal Squamous Cell Carcinoma Cell Line Ec-109,Cisplatin-induced Senescence and Senescence-related Gene Expression Profiles in#br# Human Nasopharyngeal Carcinoma Cells CNE2,Role of IL-1RAP in Gliomas Cells and Its Relationship with STAT3,Effects of MNNG on Proliferation, Cycle and Apoptosis of Kazakh Normal Esophageal Epithelial Cells
Abstract:
摘要 目的 观察黄芪多糖抑制气阴两虚Lewis肺癌荷瘤小鼠的生长、转移及对肺癌细胞周期的影响。方法 体外培养Lewis肺癌细胞，随机分为对照组和中药组，流式细胞术检测细胞周期；C57BL/6J小鼠90只，设空白组10只，余80只刨花烟熏并灌胃温热性的中药，移植Lewis肺癌实体肿瘤建立气阴两虚荷瘤小鼠模型并随机分为8组，比较各组小鼠抑瘤率及q值，计数外周血细胞及骨髓细胞，ELISA测定血清IL-2、IFN-γ、TNF-α、IL-10、HIF-1α、VEGF、MMP-2的含量。结果 黄芪多糖将Lewis 肺癌细胞阻滞于S期，随着药物浓度的增加，细胞凋亡逐渐增加；联合用药各组的抑瘤率高于黄芪多糖各组和顺铂组，q值均在0.85~1.15之间；与顺铂组比较，联合用药中、高剂量组小鼠外周血细胞及骨髓细胞的数量，IL-2、INF-γ、TNF-α均显著升高，IL-10、 HIF-1α、VEGF、MMP-2均显著降低（P<0.05, P<0.01）。结论 黄芪多糖在体外对Lewis肺癌细胞有抑制作用，体内与顺铂联合能抑制气阴两虚Lewis荷瘤小鼠肺癌细胞的生长、转移，提高外周血细胞和骨髓细胞的细胞数量，提高IL-2、INF-γ、TNF-α的含量，减少IL-10、HIF-1α、VEGF、MMP-2的含量
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