%0 Journal Article
%T 黄芪多糖对气阴两虚Lewis肺癌荷瘤小鼠肿瘤生长、转移及细胞周期的影响
%A 明海霞
%A 李杨
%A 杨玲玲
%A 白彦丽
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.0220
%X 摘要 目的 观察黄芪多糖抑制气阴两虚Lewis肺癌荷瘤小鼠的生长、转移及对肺癌细胞周期的影响。方法 体外培养Lewis肺癌细胞，随机分为对照组和中药组，流式细胞术检测细胞周期；C57BL/6J小鼠90只，设空白组10只，余80只刨花烟熏并灌胃温热性的中药，移植Lewis肺癌实体肿瘤建立气阴两虚荷瘤小鼠模型并随机分为8组，比较各组小鼠抑瘤率及q值，计数外周血细胞及骨髓细胞，ELISA测定血清IL-2、IFN-γ、TNF-α、IL-10、HIF-1α、VEGF、MMP-2的含量。结果 黄芪多糖将Lewis 肺癌细胞阻滞于S期，随着药物浓度的增加，细胞凋亡逐渐增加；联合用药各组的抑瘤率高于黄芪多糖各组和顺铂组，q值均在0.85~1.15之间；与顺铂组比较，联合用药中、高剂量组小鼠外周血细胞及骨髓细胞的数量，IL-2、INF-γ、TNF-α均显著升高，IL-10、 HIF-1α、VEGF、MMP-2均显著降低（P<0.05, P<0.01）。结论 黄芪多糖在体外对Lewis肺癌细胞有抑制作用，体内与顺铂联合能抑制气阴两虚Lewis荷瘤小鼠肺癌细胞的生长、转移，提高外周血细胞和骨髓细胞的细胞数量，提高IL-2、INF-γ、TNF-α的含量，减少IL-10、HIF-1α、VEGF、MMP-2的含量
%K 黄芪多糖
%K 气阴两虚
%K Lewis肺癌
%K 细胞周期
%K 抗瘤增效
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%U http://www.zlfzyj.com/CN/abstract/abstract9118.shtml