全部 标题 作者 关键词 摘要
DOI: 10.3971/j.issn.1000-8578.2018.17.1484
Keywords: A Case Report,Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells,Comparison of Survival Rates Based on Recommended Treatment Algorithms of BCLC or HKLC Staging Systems for Hepatocellar Carcinoma Patients,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein,TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance,Comparison of Efficiency and Safety Between Radiofrequency Ablation and Stereotactic Body Radiotherapy on Primary Small Hepatocellular Carcinoma,Effect of MicroRNA-340 on Proliferation and Apoptosis of Hepatocellular Carcinoma Cell Lines,Effect of Cyclin G1 on Radiosensitivity of Hepatocellular Carcinoma HepG2 Cells and Its Mechanism,Apoptotic Effect of Quinalizarin on Human Hepatoma Huh7 Cells and Its Antitumor Mechanism,A Meta-analysis on Relationship of Dietary Factors with Liver Cancer in Chinese Population
Full-Text Cite this paper Add to My Lib
摘要 目的 探讨靶向分子CD24单克隆抗体3E10调节肝癌HuH-7细胞对化疗药顺铂敏感度的作用及其分子机制。方法 Western blot和流式细胞术检测抗CD24抗体3E10的特异性；Annexin V-FITC/PI双染色检测细胞凋亡；Real-time PCR和Western blot检测耐药基因和肿瘤干细胞特性基因的表达水平；Western blot检测JAK/STAT3和PI3K/AKT信号通路的活性水平。结果 3E10可有效识别CD24蛋白及HuH-7细胞。3E10显著增强HuH-7细胞对顺铂的敏感度（P<0.05），抑制率由（10.3±3.0）%提高至（34.4±10.8）%。3E10显著降低HuH-7细胞耐药基因ABCB1、ABCB5、ABCC1和肿瘤干性基因NANOG、CD24的表达水平及干性基因β-catenin的磷酸化水平（P<0.05）。3E10显著降低STAT3和AKT的磷酸化水平（P<0.05）。结论 靶向CD24分子3E10通过降低HuH-7细胞的耐药性、肿瘤干性和JAK/STAT3、PI3K/AKT信号通路活性来增强HuH-7细胞对化疗药顺铂的敏感度
Full-Text
Contact Us
service@oalib.com
QQ:3279437679
WhatsApp +8615387084133
