%0 Journal Article
%T 靶向CD24分子3E10增强肝癌HuH-7细胞的化疗敏感度
%A 于宝丹
%A 吕萍
%A 方昶
%A 程庆
%A 黄泽坚
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1484
%X 摘要 目的 探讨靶向分子CD24单克隆抗体3E10调节肝癌HuH-7细胞对化疗药顺铂敏感度的作用及其分子机制。方法 Western blot和流式细胞术检测抗CD24抗体3E10的特异性；Annexin V-FITC/PI双染色检测细胞凋亡；Real-time PCR和Western blot检测耐药基因和肿瘤干细胞特性基因的表达水平；Western blot检测JAK/STAT3和PI3K/AKT信号通路的活性水平。结果 3E10可有效识别CD24蛋白及HuH-7细胞。3E10显著增强HuH-7细胞对顺铂的敏感度（P<0.05），抑制率由（10.3±3.0）%提高至（34.4±10.8）%。3E10显著降低HuH-7细胞耐药基因ABCB1、ABCB5、ABCC1和肿瘤干性基因NANOG、CD24的表达水平及干性基因β-catenin的磷酸化水平（P<0.05）。3E10显著降低STAT3和AKT的磷酸化水平（P<0.05）。结论 靶向CD24分子3E10通过降低HuH-7细胞的耐药性、肿瘤干性和JAK/STAT3、PI3K/AKT信号通路活性来增强HuH-7细胞对化疗药顺铂的敏感度
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%U http://www.zlfzyj.com/CN/abstract/abstract9206.shtml