隐丹参酮诱导人食管癌HCE-4细胞凋亡的机制
DOI: 10.3971/j.issn.1000-8578.2018.17.1582
Keywords: 隐丹参酮,食管癌,HCE-4细胞,MAPKs信号通路,AKT信号通路,活性氧,Effect of Selective Increase of Single Dose of PTV on Prognosis of Patients with Esophageal Cancer,Silence of STAT3 Enhances SNX-2112-induced Apoptosis of Esophageal Cancer Stem Cells,Microarray Analysis of Radioresistance-associated Genes in Esophageal Squamous Cell Carcinoma,Effect of miR-7 Overexpression on Biological Behavior of Colon Carcinoma HCT-116 Cells,Application of COMPASS 3D Dose Validation System in Volumetric Modulated Arc Therapy of Esophageal Cancer,Research Progress of Long Non-coding RNA in Esophageal Cancer,MCM7 Knockdown Inhibits Migration and Invasion While Induces Apoptosis of Cutaneous Malignant Melanoma Cells Through AKT Signaling Pathway,NOTCH Signaling Pathway Regulates Glioma Stem-like Cell Proliferation and Selfrenewal Abilities via PI3K/AKT Activity,Effects of Aloin on Proliferation, Apoptosis and Invasion of Esophageal Cancer KESY70 Cell Line,Berberine Inhibits Proliferation and Induces Apoptosis of Esophageal Cancer Cells via Inactivation of IGF-1R Signaling Pathway,Prognosis of Stage ⅡB-Ⅲ Thoracic Esophageal Carcinoma Patients Treated with Prophylactic After Radical Esophagectomy,Clinical Characteristics and Prognostic Factors of 35 Patients with Esophagus Adenoid Cystic Carcinoma Patients,Impact of SLC39A5 Knockout on Establishment of Esophageal Cancer Model Induced by 4-NQO in C57BL/6 Mice,Preoperative Platelet-to-lymphocyte Ratio and Neutrophil-to-lymphocyte Ratio for Predicting Prognosis of Esophageal Cancer Patients,Clinical Comparison of Minimally Invasive Esophagectomy Versus Open Esophagectomy on Elderly Esophageal Carcinoma Patients (≥70 Years Old)
Abstract:
摘要 目的 探讨隐丹参酮对食管癌HCE-4细胞的药效和药理机制。方法 MTT比色法检测隐丹参酮对人食管癌HCE-4和TE-2细胞的杀伤作用。倒置显微镜观察隐丹参酮处理后HCE-4细胞形态学变化。Annexin V-FITC/PI双染法和流式细胞术检测隐丹参酮对HCE-4细胞的诱导凋亡作用、活性氧水平及加入活性氧清除剂NAC后细胞凋亡情况。Western blot检测细胞凋亡相关蛋白的表达量。结果 MTT比色法检测结果显示隐丹参酮对HCE-4和TE-2细胞均具有良好的杀伤作用。经隐丹参酮处理后，HCE-4细胞呈现细胞核固缩、细胞变圆、悬浮细胞增多的现象。Annexin V-FITC/PI双染法和流式细胞术检测结果表明隐丹参酮可以诱导HCE-4细胞发生凋亡，增加细胞内活性氧的水平，预处理NAC后，隐丹参酮的诱导凋亡作用被抑制。Western blot检测结果显示促凋亡蛋白p-JNK、p-p38、Bad、Caspase-3及PARP表达量升高，抗凋亡蛋白p-ERK、p-AKT及Bcl-2表达量降低。结论 隐丹参酮对人食管癌HCE-4细胞具有良好的杀伤作用，其机制可能是隐丹参酮上调ROS的水平，调控MAPK和AKT信号途径，进而诱导食管癌HCE-4细胞发生凋亡
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