%0 Journal Article
%T 隐丹参酮诱导人食管癌HCE-4细胞凋亡的机制
%A 张翼
%A 徐宛婷
%A 朴仙姬
%A 李金钱
%A 王加茹
%A 王浩
%A 罗英花
%A 金成浩
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1582
%X 摘要 目的 探讨隐丹参酮对食管癌HCE-4细胞的药效和药理机制。方法 MTT比色法检测隐丹参酮对人食管癌HCE-4和TE-2细胞的杀伤作用。倒置显微镜观察隐丹参酮处理后HCE-4细胞形态学变化。Annexin V-FITC/PI双染法和流式细胞术检测隐丹参酮对HCE-4细胞的诱导凋亡作用、活性氧水平及加入活性氧清除剂NAC后细胞凋亡情况。Western blot检测细胞凋亡相关蛋白的表达量。结果 MTT比色法检测结果显示隐丹参酮对HCE-4和TE-2细胞均具有良好的杀伤作用。经隐丹参酮处理后，HCE-4细胞呈现细胞核固缩、细胞变圆、悬浮细胞增多的现象。Annexin V-FITC/PI双染法和流式细胞术检测结果表明隐丹参酮可以诱导HCE-4细胞发生凋亡，增加细胞内活性氧的水平，预处理NAC后，隐丹参酮的诱导凋亡作用被抑制。Western blot检测结果显示促凋亡蛋白p-JNK、p-p38、Bad、Caspase-3及PARP表达量升高，抗凋亡蛋白p-ERK、p-AKT及Bcl-2表达量降低。结论 隐丹参酮对人食管癌HCE-4细胞具有良好的杀伤作用，其机制可能是隐丹参酮上调ROS的水平，调控MAPK和AKT信号途径，进而诱导食管癌HCE-4细胞发生凋亡
%K 隐丹参酮
%K 食管癌
%K HCE-4细胞
%K MAPKs信号通路
%K AKT信号通路
%K 活性氧
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%U http://www.zlfzyj.com/CN/abstract/abstract9205.shtml