miR-205对胃癌细胞侵袭迁移的调控作用及其潜在机制
DOI: 10.3971/j.issn.1000-8578.2018.17.1217
Keywords: miR-205,上皮间质转化,胃癌,侵袭,迁移,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
Abstract:
摘要 目的 探讨miR-205对胃癌HGC27细胞侵袭、迁移的作用及其机制。方法 实时荧光定量PCR法测定4种不同胃癌细胞（AGS、MKN74、HGC27、SGC7901）中miR-205的表达情况。体外培养的HGC27细胞通过转染miR-205 mimic上调细胞中miR-205的表达，划痕实验和Transwell实验观察其侵袭和迁移能力；Western blot检测上皮间质转化（epithelial-mesenchymal transition, EMT）进程及相关信号通路的活性。结果 miR-205在4种胃癌细胞中均呈低表达（均P<0.05）。过表达miR-205后，HGC27细胞的迁移率由（54.7±4.1）%降低至（34.1±4.5）% （P=0.005）；细胞的侵袭率由（52.8±6.3）%降低至（32.2±4.9）%（P=0.001）。此外，过表达miR-205之后，胃癌细胞中上皮细胞标志物E-cadherin、β-catenin蛋白表达显著上升（P=0.002, P=0.003），而间质细胞标志物N-cadherin、vimentin的蛋白表达显著下降（P=0.005, P=0.004），Notch和snail的蛋白表达水平也显著降低（P=0.002, P=0.003）。结论 miR-205在胃癌细胞中低表达，且与胃癌细胞的侵袭和迁移密切相关；其分子机制可能与抑制EMT及Notch/snail信号通路有关
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