%0 Journal Article
%T miR-205对胃癌细胞侵袭迁移的调控作用及其潜在机制
%A 崔华胜
%A 王慧敏
%A 王玉霞
%A 王芳
%A 申绯翡
%A 索生红
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1217
%X 摘要 目的 探讨miR-205对胃癌HGC27细胞侵袭、迁移的作用及其机制。方法 实时荧光定量PCR法测定4种不同胃癌细胞（AGS、MKN74、HGC27、SGC7901）中miR-205的表达情况。体外培养的HGC27细胞通过转染miR-205 mimic上调细胞中miR-205的表达，划痕实验和Transwell实验观察其侵袭和迁移能力；Western blot检测上皮间质转化（epithelial-mesenchymal transition, EMT）进程及相关信号通路的活性。结果 miR-205在4种胃癌细胞中均呈低表达（均P<0.05）。过表达miR-205后，HGC27细胞的迁移率由（54.7±4.1）%降低至（34.1±4.5）% （P=0.005）；细胞的侵袭率由（52.8±6.3）%降低至（32.2±4.9）%（P=0.001）。此外，过表达miR-205之后，胃癌细胞中上皮细胞标志物E-cadherin、β-catenin蛋白表达显著上升（P=0.002, P=0.003），而间质细胞标志物N-cadherin、vimentin的蛋白表达显著下降（P=0.005, P=0.004），Notch和snail的蛋白表达水平也显著降低（P=0.002, P=0.003）。结论 miR-205在胃癌细胞中低表达，且与胃癌细胞的侵袭和迁移密切相关；其分子机制可能与抑制EMT及Notch/snail信号通路有关
%K miR-205
%K 上皮间质转化
%K 胃癌
%K 侵袭
%K 迁移
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%K Effects of Sanguinarine on Invasion
%K Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP
%K Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients
%K Effect of Down-regulation of HOXB7 Expression on Proliferation
%K Migration and Invasion Abilities of Hepatoma Cells
%K Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性
%K Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer
%K Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
%U http://www.zlfzyj.com/CN/abstract/abstract9149.shtml