HPV16-E6上调MIF对宫颈癌细胞增殖和凋亡的影响
DOI: 10.3971/j.issn.1000-8578.2018.17.1406
Keywords: 宫颈癌,巨噬细胞移动抑制因子（MIF）,人乳头瘤病毒16型的致癌基因E6（HPV16-E6）,凋亡,Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism,Anti-tumor Effects of Chlorogenic Acid on Human Glioblastoma Cell Lines U251 and Related Pro-apoptotic Mechanism,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma,RNA PolymeraseⅠInhibitor Regulates Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb,Knockdown of EN2 Induces Apoptosis and Enhances PTEN Protein Expression in Hepatocellular Carcinoma Cells,Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells
Abstract:
摘要 目的 检测人乳头瘤病毒16型的致癌基因E6（HPV16-E6）通过巨噬细胞移动抑制因子（MIF）对宫颈癌细胞增殖和凋亡的影响。方法 用HPV16-E6过表达和干扰质粒转染能分泌MIF的人宫颈癌细胞C33A、SiHa和Caski。Western blot和荧光定量PCR法检测MIF蛋白和mRNA表达，并用ELISA法检测培养上清液中MIF蛋白量；将转染后的宫颈癌细胞与人巨噬细胞非接触性共培养，检测巨噬细胞中MIF表达；C33A转染HPV16-E6后加或不加MIF抑制剂，CCK-8法和流式细胞术检测细胞增殖、周期和凋亡情况；采用Pearson相关分析法分析HPV16-E6和MIF间的关系、Kaplan-Meier分析HPV16-E6对患者生存率的影响。结果 癌细胞、上清液和巨噬细胞中MIF表达随HPV16-E6表达而上调（P<0.05）；HPV16-E6过表达可诱导C33A细胞增殖、减少G0/G1期细胞和抑制细胞凋亡；抑制MIF后，细胞增殖率下降、 凋亡率增加（P<0.05）；HPV16-E6表达与MIF正相关（P<0.05），Kaplan-Meier分析显示HPV16-E6表达与患者总生存率和无进展生存率有关（P<0.05）。结论 HPV16-E6可诱导宫颈癌细胞及其微环境中巨噬细胞MIF表达，并可通过MIF诱导宫颈癌细胞增殖和抑制细胞凋亡而影响宫颈癌的预后
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