%0 Journal Article
%T HPV16-E6上调MIF对宫颈癌细胞增殖和凋亡的影响
%A 宋紫烨
%A 徐梦菲
%A 李蓁
%A 葛彩云
%A 蔡红兵
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1406
%X 摘要 目的 检测人乳头瘤病毒16型的致癌基因E6（HPV16-E6）通过巨噬细胞移动抑制因子（MIF）对宫颈癌细胞增殖和凋亡的影响。方法 用HPV16-E6过表达和干扰质粒转染能分泌MIF的人宫颈癌细胞C33A、SiHa和Caski。Western blot和荧光定量PCR法检测MIF蛋白和mRNA表达，并用ELISA法检测培养上清液中MIF蛋白量；将转染后的宫颈癌细胞与人巨噬细胞非接触性共培养，检测巨噬细胞中MIF表达；C33A转染HPV16-E6后加或不加MIF抑制剂，CCK-8法和流式细胞术检测细胞增殖、周期和凋亡情况；采用Pearson相关分析法分析HPV16-E6和MIF间的关系、Kaplan-Meier分析HPV16-E6对患者生存率的影响。结果 癌细胞、上清液和巨噬细胞中MIF表达随HPV16-E6表达而上调（P<0.05）；HPV16-E6过表达可诱导C33A细胞增殖、减少G0/G1期细胞和抑制细胞凋亡；抑制MIF后，细胞增殖率下降、 凋亡率增加（P<0.05）；HPV16-E6表达与MIF正相关（P<0.05），Kaplan-Meier分析显示HPV16-E6表达与患者总生存率和无进展生存率有关（P<0.05）。结论 HPV16-E6可诱导宫颈癌细胞及其微环境中巨噬细胞MIF表达，并可通过MIF诱导宫颈癌细胞增殖和抑制细胞凋亡而影响宫颈癌的预后
%K 宫颈癌
%K 巨噬细胞移动抑制因子（MIF）
%K 人乳头瘤病毒16型的致癌基因E6（HPV16-E6）
%K 凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract9190.shtml