FGF3和FGF10对人卵巢癌SKOV3细胞迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系
DOI: 10.3971/j.issn.1000-8578.2018.18.0484
Keywords: FGF3,FGF10,卵巢癌,迁移,侵袭,Wnt/β-catenin信号通路,小干扰RNA,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Efficacy of 93 Cases of Recurrent Ovarian Cancer,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Adverse Reaction of HIPEC Combined with Intravenous Chemotherapy on Ovarian Cancer Patients,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Progress on Relationship of Meat Consumption with Incidence and Prognosis of Ovarian Cancer,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
Abstract:
摘要 目的 探讨FGF3和FGF10对人卵巢癌SKOV3细胞株迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系。方法 体外培养人卵巢癌SKOV3细胞，采用人工合成siRNA干扰细胞的FGF3和FGF10基因，运用Real-time PCR和Western blot分别从mRNA水平和蛋白水平检测干扰效果。Transwell检测FGF3和FGF10蛋白表达下调后细胞迁移和侵袭能力的变化。采用LiCl激活Wnt/β-catenin信号通路，运用Real-time PCR检测FGF3和FGF10基因的变化。结果 FGF3和FGF10基因沉默组细胞迁移和侵袭能力与阴性对照（NC）组细胞、未处理组SKOV3细胞相比明显减弱（P<0.05），NC组与未处理组SKOV3细胞迁移和侵袭能力差异无统计学意义（P>0.05）。 激活Wnt/β-catenin信号通路后，FGF3和FGF10基因水平增高。结论 FGF3和FGF10基因受Wnt/β-catenin信号通路的调控，促进人卵巢癌SKOV3细胞的迁移和侵袭
Full-Text
