%0 Journal Article
%T FGF3和FGF10对人卵巢癌SKOV3细胞迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系
%A 朱克修
%A 王佳
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0484
%X 摘要 目的 探讨FGF3和FGF10对人卵巢癌SKOV3细胞株迁移和侵袭的影响及其与Wnt/β-catenin信号通路的关系。方法 体外培养人卵巢癌SKOV3细胞，采用人工合成siRNA干扰细胞的FGF3和FGF10基因，运用Real-time PCR和Western blot分别从mRNA水平和蛋白水平检测干扰效果。Transwell检测FGF3和FGF10蛋白表达下调后细胞迁移和侵袭能力的变化。采用LiCl激活Wnt/β-catenin信号通路，运用Real-time PCR检测FGF3和FGF10基因的变化。结果 FGF3和FGF10基因沉默组细胞迁移和侵袭能力与阴性对照（NC）组细胞、未处理组SKOV3细胞相比明显减弱（P<0.05），NC组与未处理组SKOV3细胞迁移和侵袭能力差异无统计学意义（P>0.05）。 激活Wnt/β-catenin信号通路后，FGF3和FGF10基因水平增高。结论 FGF3和FGF10基因受Wnt/β-catenin信号通路的调控，促进人卵巢癌SKOV3细胞的迁移和侵袭
%K FGF3
%K FGF10
%K 卵巢癌
%K 迁移
%K 侵袭
%K Wnt/β-catenin信号通路
%K 小干扰RNA
%K miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17
%K Efficacy of 93 Cases of Recurrent Ovarian Cancer
%K Effects of Sanguinarine on Invasion
%K Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Adverse Reaction of HIPEC Combined with Intravenous Chemotherapy on Ovarian Cancer Patients
%K Effect of Down-regulation of HOXB7 Expression on Proliferation
%K Migration and Invasion Abilities of Hepatoma Cells
%K Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Progress on Relationship of Meat Consumption with Incidence and Prognosis of Ovarian Cancer
%K Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer
%K Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
%U http://www.zlfzyj.com/CN/abstract/abstract9293.shtml