δ-三烯生育酚通过上调MicroRNA-34a抑制宫颈癌细胞增殖
DOI: 10.3971/j.issn.1000-8578.2018.18.0523
Keywords: 宫颈癌,δ-三烯生育酚,miR-34a,增殖,CCND1,Blockade of Radiation-induced EGFR Nuclear Transport Enhances Radiosensitivity of Human Cervical Cancer,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Comparison of Survival and Postoperative Complication of Stage Ⅰb2-Ⅱa2 Cervical Cancer Patients Among Three Kinds of Therapeutic Regimens,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Downregulation of Skp2 Expression Inhibits Malignant Phenotype of Osteosarcoma U2OS Cells,Effects of NDUFA4 Overexpression on Growth of Human Colon Cancer Cells in vitro,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Expression and Biological Functions of Sestrin2 in Nasopharyngeal Carcinoma,RNA PolymeraseⅠInhibitor Regulates Proliferation, Invasion and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE-1 by NF-κb,Effect of TAK-242 on Proliferation and Apoptosis of Human Multiple Myeloma Cells
Abstract:
摘要 目的 探究δ-三烯生育酚对HeLa宫颈癌细胞增殖的影响以及microRNA-34a（miR-34a）是否介导这一过程。方法 用荧光定量PCR方法检测miR-34a和细胞增殖关键基因表达的变化，CCK-8检测细胞活力变化，Western blot检测细胞增殖关键基因蛋白水平的变化，流式细胞术检测细胞周期变化；并利用miR-34a模拟剂和抑制剂，探究其对细胞增殖的影响。结果 δ-三烯生育酚处理HeLa宫颈癌细胞36 h显著降低细胞活力并呈剂量依赖效应。用20 μmol/L δ-三烯生育酚处理细胞36 h显著上调miR-34a表达，显著下调细胞周期关键基因CCND1，同时S细胞比例显著下降。细胞转染miR-34a模拟剂后，CCND1表达和细胞活力都下降；而转染miR-34a抑制剂则显著减弱δ-三烯生育酚的增殖抑制作用。结论 δ-三烯生育酚通过上调miR-34a抑制宫颈癌细胞增殖
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