%0 Journal Article
%T δ-三烯生育酚通过上调MicroRNA-34a抑制宫颈癌细胞增殖
%A 余瑛
%A 刘联斌
%A 李荣
%A 王琪
%A 罗德平
%A 黄晶
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0523
%X 摘要 目的 探究δ-三烯生育酚对HeLa宫颈癌细胞增殖的影响以及microRNA-34a（miR-34a）是否介导这一过程。方法 用荧光定量PCR方法检测miR-34a和细胞增殖关键基因表达的变化，CCK-8检测细胞活力变化，Western blot检测细胞增殖关键基因蛋白水平的变化，流式细胞术检测细胞周期变化；并利用miR-34a模拟剂和抑制剂，探究其对细胞增殖的影响。结果 δ-三烯生育酚处理HeLa宫颈癌细胞36 h显著降低细胞活力并呈剂量依赖效应。用20 μmol/L δ-三烯生育酚处理细胞36 h显著上调miR-34a表达，显著下调细胞周期关键基因CCND1，同时S细胞比例显著下降。细胞转染miR-34a模拟剂后，CCND1表达和细胞活力都下降；而转染miR-34a抑制剂则显著减弱δ-三烯生育酚的增殖抑制作用。结论 δ-三烯生育酚通过上调miR-34a抑制宫颈癌细胞增殖
%K 宫颈癌
%K δ-三烯生育酚
%K miR-34a
%K 增殖
%K CCND1
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%U http://www.zlfzyj.com/CN/abstract/abstract9292.shtml