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DOI: 10.3971/j.issn.1000-8578.2018.18.0088
Keywords: A Predictor of Risk and Prognosis of Breast Cancer,Tanshinone ⅡA Enhances Chemosensitivity of Breast Cancer Cells to Doxorubicin and Related Mechanism,In Situ Hybridization Status of 568 Cases of HER2 Equivocal Breast Cancer,Apatinib Suppresses Proliferation and Induced Apoptosis of Human Breast Cancer Cell Line MDA-MB-231 Through Glycolytic Inhibition,Progress of Whole Exon Sequencing in Pathogenesis and Diagnosis of Breast Cancer,Feedback Activation of STAT3 Confers Resistance of HER2-positive Breast Cancer Cells to Lapatinib,Influence of NRP-1 Monoclonal Antibody on Growth of Breast Cancer Xenografts in Nude Mouse,Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells,Breast Metastasis of Primary Pulmonary Adenocarcinoma: A Report of Two Cases and Literature Review
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摘要 目的 探讨HIF-2α对乳腺癌MCF-7细胞增殖、侵袭及成瘤能力的影响。方法 利用HIF-2αORF/shRNA慢病毒颗粒分别感染乳腺癌MCF-7细胞，筛选构建MCF-7 HIF-2α上调或下调的稳定细胞株；利用Real-time PCR和Western blot方法分别检测HIF-2α mRNA和蛋白的表达水平；CCK-8方法检测细胞增殖活力；Transwell小室侵袭实验检测细胞的侵袭能力变化；平板克隆形成实验检测细胞克隆形成能力。结果 HIF-2α shRNA质粒转染MCF-7细胞后HIF-2α的表达水平均显著下降；HIF-2α ORF质粒转染MCF-7细胞后HIF-2α的表达水平显著升高。CCK-8检测结果显示：HIF-2α上调后MCF-7细胞增殖活力增强；Transwell小室实验结果显示：HIF-2α上调后MCF-7细胞侵袭能力增强；平板克隆形成实验结果显示：HIF-2α上调后MCF-7细胞克隆形成能力增强。而HIF-2α下调MCF-7细胞具有相反趋势。结论 HIF-2α过表达能够增强MCF-7细胞增殖活力、促进肿瘤细胞侵袭和克隆形成
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