%0 Journal Article
%T HIF-2α对乳腺癌MCF-7细胞生物学功能的影响
%A 刘宇琼
%A 李娜
%A 李惠翔
%A 黄会粉
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0088
%X 摘要 目的 探讨HIF-2α对乳腺癌MCF-7细胞增殖、侵袭及成瘤能力的影响。方法 利用HIF-2αORF/shRNA慢病毒颗粒分别感染乳腺癌MCF-7细胞，筛选构建MCF-7 HIF-2α上调或下调的稳定细胞株；利用Real-time PCR和Western blot方法分别检测HIF-2α mRNA和蛋白的表达水平；CCK-8方法检测细胞增殖活力；Transwell小室侵袭实验检测细胞的侵袭能力变化；平板克隆形成实验检测细胞克隆形成能力。结果 HIF-2α shRNA质粒转染MCF-7细胞后HIF-2α的表达水平均显著下降；HIF-2α ORF质粒转染MCF-7细胞后HIF-2α的表达水平显著升高。CCK-8检测结果显示：HIF-2α上调后MCF-7细胞增殖活力增强；Transwell小室实验结果显示：HIF-2α上调后MCF-7细胞侵袭能力增强；平板克隆形成实验结果显示：HIF-2α上调后MCF-7细胞克隆形成能力增强。而HIF-2α下调MCF-7细胞具有相反趋势。结论 HIF-2α过表达能够增强MCF-7细胞增殖活力、促进肿瘤细胞侵袭和克隆形成
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%U http://www.zlfzyj.com/CN/abstract/abstract9243.shtml