长链非编码RNA ZNF667-AS1在食管鳞状细胞癌组织中的表达及其DNA甲基化状态的研究
DOI: 10.3971/j.issn.1000-8578.2018.18.0586
Keywords: 食管鳞状细胞癌,ZNF667-AS1,长链非编码RNA,DNA甲基化,Research Progress of RNA Molecules in Thyroid Cancer,Effects of Long Non-coding RNA-MALAT1 on Proliferation and Apoptosis of Prostate Cancer Cells,Down-regulation of VRK1 Expression by siRNA Suppresses BANF1 Expression and Proliferation and Migration Abilities of Esophageal Squamous Cell Carcinoma Cells,Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma,LncRNA AC009336.24 Expression in Small Cell Lung Cancer and Its Relationship with Chemotherapy Resistance,Relationship of LncRNA AC010145.4 Expression with Prognosis and Chemoresistance of Small Cell Lung Cancer,Correlation of ChREBP mRNA Expression and Its CpG Island Methylation in Human Hepatocellular Carcinoma,Effect of Long Non-coding RNA LOC100294362 on Proliferation and Invasion of Breast Cancer Cells,Significance of Tumor Suppressor Gene TCF21 Promoter Methylation for Diagnosis and Prognosis of Renal Cell Carcinoma,LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma,Expression of EZH2 and H3K27me3 in Esophageal Squamous Cell Carcinoma and Their Significance,Association Between Long Non-coding RNA and Cervical Cancer,Metastasis?associated Lung Adenocarcinoma Transcript1(MALAT1) Expression in Middle Thoracic Esophageal Squamous Cell Cancer Tissues and Its Relationship with Prognosis,Progress of Epigenetic Regulation of H3K27me3 and EZH2 in B-cell Non-Hodgkin's Lymphoma,Support Vector Machine Predicts Survival of Esophageal Squamous Cell Carcinoma Patients
Abstract:
摘要 目的 检测长链非编码RNA ZNF667-AS1在食管鳞状细胞癌（esophageal squamous cell carcinoma, ESCC）中的表达及甲基化状态。方法 应用qPCR和MSP法检测DNA甲基化转移酶抑制剂5-Aza-dC处理前后食管癌细胞系（Kyse170、Eca109、TE1和TE13）、ESCC及相应癌旁正常组织中ZNF667-AS1基因的表达，及其CpG岛三个区域的甲基化状态。结果 在5-Aza-dC处理后的四株细胞系中，ZNF667-AS1基因的表达均增高，且其CpG岛三个区域甲基化程度均降低。ZNF667-AS1基因在ESCC组织中的表达显著低于相应癌旁正常组织，且该基因CpG岛远端启动子区及第一外显子区甲基化率在ESCC与相应癌旁正常组织中的差异均具有统计学意义（均P<0.05）。ESCC组织中CpG岛近端启动子区甲基化率显著高于相应癌旁正常组织，并与组织分化程度、TNM分期密切相关，ZNF667-AS1基因CpG岛近端启动子区发生甲基化的ESCC组织中，低表达例数高于高表达例数，差异具有统计学意义。结论 ZNF667-AS1基因在食管癌细胞系和ESCC组织中的低表达与ESCC的发生发展密切相关，且其近端启动子区的高甲基化状态可能是导 致其表达沉默的机制之一
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