%0 Journal Article
%T 长链非编码RNA ZNF667-AS1在食管鳞状细胞癌组织中的表达及其DNA甲基化状态的研究
%A 任利兵
%A 吴璇
%A 康小亮
%A 梁佳
%A 董稚明
%A 邝钢
%A 郭炜
%A 郭艳丽
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0586
%X 摘要 目的 检测长链非编码RNA ZNF667-AS1在食管鳞状细胞癌（esophageal squamous cell carcinoma, ESCC）中的表达及甲基化状态。方法 应用qPCR和MSP法检测DNA甲基化转移酶抑制剂5-Aza-dC处理前后食管癌细胞系（Kyse170、Eca109、TE1和TE13）、ESCC及相应癌旁正常组织中ZNF667-AS1基因的表达，及其CpG岛三个区域的甲基化状态。结果 在5-Aza-dC处理后的四株细胞系中，ZNF667-AS1基因的表达均增高，且其CpG岛三个区域甲基化程度均降低。ZNF667-AS1基因在ESCC组织中的表达显著低于相应癌旁正常组织，且该基因CpG岛远端启动子区及第一外显子区甲基化率在ESCC与相应癌旁正常组织中的差异均具有统计学意义（均P<0.05）。ESCC组织中CpG岛近端启动子区甲基化率显著高于相应癌旁正常组织，并与组织分化程度、TNM分期密切相关，ZNF667-AS1基因CpG岛近端启动子区发生甲基化的ESCC组织中，低表达例数高于高表达例数，差异具有统计学意义。结论 ZNF667-AS1基因在食管癌细胞系和ESCC组织中的低表达与ESCC的发生发展密切相关，且其近端启动子区的高甲基化状态可能是导 致其表达沉默的机制之一
%K 食管鳞状细胞癌
%K ZNF667-AS1
%K 长链非编码RNA
%K DNA甲基化
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%U http://www.zlfzyj.com/CN/abstract/abstract9294.shtml