shRNA干扰半胱氨酸蛋白酶抑制剂C抑制非小细胞肺癌A549细胞侵袭和迁移
DOI: 10.3971/j.issn.1000-8578.2018.18.0802
Keywords: 半胱氨酸蛋白酶抑制剂,迁移,侵袭,非小细胞癌,miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17,Effects of Sanguinarine on Invasion, Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Effect of Down-regulation of HOXB7 Expression on Proliferation, Migration and Invasion Abilities of Hepatoma Cells,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer,Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein,Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells,Role of Gadd45 Protein Family in Tumorigenesis and Development,miR-145 Attenuates Migration and Invasion of Non-small Cell Lung Cancer A549 Cells by Targeting AP4,miR-198 Targets MAPK1 to Regulate Proliferation, Apoptosis and Invasion of Cervical Cancer HeLa Cells
Abstract:
摘要 目的 研究半胱氨酸蛋白酶抑制剂C（cystatin C, Cys C）表达对NSCLC A549细胞的生长、侵袭和转移的作用及其潜在的分子机制。方法 采用短发夹RNA（sh-RNA）干扰沉默Cys C表达，构建sh-Cys C载体，并转染人肺腺癌A549细胞中，筛选sh-Cys C稳转子。用CCK-8法检测细胞增殖；划痕实验检测细胞迁移；Transwell实验检测细胞侵袭。此外，还采用Western blot检测侵袭和迁移关键蛋白表达的变化。结果 shRNA干扰Cys C，明显下调Cys C的表达；与对照组相比，发现shRNA介导的Cys C沉默显著抑制A549细胞体外增殖、迁移和侵袭。此外，还发现Cys C的下调可以显著抑制基质金属蛋白酶（MMP9、MMP14）和血管内皮生长因子（VEGF）的表达。结论 shRNA干扰半胱氨酸蛋白酶抑制剂C，明显下调Cys C的表达，Cys C的下调可抑制非小细胞肺癌A549细胞的侵袭和迁移
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