%0 Journal Article
%T shRNA干扰半胱氨酸蛋白酶抑制剂C抑制非小细胞肺癌A549细胞侵袭和迁移
%A 刘仕强
%A 汪华
%A 范丽丹
%A 陈旭
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0802
%X 摘要 目的 研究半胱氨酸蛋白酶抑制剂C（cystatin C, Cys C）表达对NSCLC A549细胞的生长、侵袭和转移的作用及其潜在的分子机制。方法 采用短发夹RNA（sh-RNA）干扰沉默Cys C表达，构建sh-Cys C载体，并转染人肺腺癌A549细胞中，筛选sh-Cys C稳转子。用CCK-8法检测细胞增殖；划痕实验检测细胞迁移；Transwell实验检测细胞侵袭。此外，还采用Western blot检测侵袭和迁移关键蛋白表达的变化。结果 shRNA干扰Cys C，明显下调Cys C的表达；与对照组相比，发现shRNA介导的Cys C沉默显著抑制A549细胞体外增殖、迁移和侵袭。此外，还发现Cys C的下调可以显著抑制基质金属蛋白酶（MMP9、MMP14）和血管内皮生长因子（VEGF）的表达。结论 shRNA干扰半胱氨酸蛋白酶抑制剂C，明显下调Cys C的表达，Cys C的下调可抑制非小细胞肺癌A549细胞的侵袭和迁移
%K 半胱氨酸蛋白酶抑制剂
%K 迁移
%K 侵袭
%K 非小细胞癌
%K miR-601 Suppresses Migration and Invasion of Non-small Cell lung Cancer Cells by Targeting MMP-17
%K Effects of Sanguinarine on Invasion
%K Migration and Wnt/β-catenin Signaling Pathway of Lung Adenocarcinoma Cells
%K Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells
%K Effect of Down-regulation of HOXB7 Expression on Proliferation
%K Migration and Invasion Abilities of Hepatoma Cells
%K Effects of miR-144 on Proliferation
%K Migration
%K Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells
%K Effect of ANLN Gene Silencing on Invasion
%K Migration
%K Proliferation and Apoptosis of Gastric Cancer Cells
%K G6PD对大肠癌细胞生长侵袭的影响及其与HKⅡ的相关性
%K Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells
%K Effect of FBXL20 on Proliferation
%K Migration and Invasion of Gastric Cancer Cells
%K Tumor-associated Macrophages Promote Invasion and Metastasis of Cervical Cancer
%K Ampelopsis Regulates Invasive Activity and Invasion Gene Expression of Hepatocellular Carcinoma HepG2 Cell Line Through Dermcidin Protein
%K Expression of Circular RNA ciRS-7 and Its Effect on Invasion and Migration of Triplenegative reast Cancer Cells
%K Role of Gadd45 Protein Family in Tumorigenesis and Development
%K miR-145 Attenuates Migration and Invasion of Non-small Cell Lung Cancer A549 Cells by Targeting AP4
%K miR-198 Targets MAPK1 to Regulate Proliferation
%K Apoptosis and Invasion of Cervical Cancer HeLa Cells
%U http://www.zlfzyj.com/CN/abstract/abstract9290.shtml