过表达SHP-1对人慢性髓系白血病急变细胞系K562生物学特征的影响
DOI: 10.3971/j.issn.1000-8578.2015.10.008
Keywords: SHP-1基因,K562细胞,凋亡,增殖,细胞周期,集落形成,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Progress of Relationships of Calprotectin with Tumours,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Expression of Toll-like Receptor 4 in Lymphoma Cell Lines and Its Effect on Cells Proliferation and Drug Resistance,GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP,Effect of Terpinen-4-ol on Proliferation and Apoptosis of Human Multiple Myeloma Cells U266
Abstract:
摘要 目的 探讨SHP-1基因对K562细胞系增殖、凋亡、细胞周期分布和集落形成的影响。方法 将携带SHP-1基因和绿色荧光蛋白（EGFP）基因的慢病毒表达载体转染K562细胞，SYBR Green荧光定量PCR和Western blot法检测转染细胞SHP-1 mRNA和蛋白的表达情况；CCK-8法检测细胞增殖；流式细胞术检测细胞凋亡率和细胞周期分布；光学显微镜和电子显微镜下观察细胞形态；TUNEL法检测细胞凋亡；培养细胞集落并比较不同组间集落形成的数量和大小。结果 培养第3天，K562SHP-1细胞的OD值为（0.35±0.02），K562EGFP细胞的OD值为（0.47±0.05），两者差异有统计学意义（P=0.011）。随着培养天数的延长，与K562EGFP细胞相比K562SHP-1细胞出现G0/G1期细胞比例增加和G2/M+S期细胞比例降低及凋亡增加，培养第5天K562SHP-1细胞G0/G1期比例为（56.37±2.27）、凋亡率为（26.13±1.16），K562EGFP细胞G0/G1期比例为（31.67±3.21），凋亡率为（9.00±1.22），两者比较差有统计学意义（P=0.000）。K562SHP-1细胞集落形成能力（26.3±5.2）较K562EGFP细胞（54.7±8.6）有所降低（P=0.000）；K562SHP-1细胞BCR-ABL1蛋白的表达水平（0.78±0.15）低于K562EGFP细胞（1.27± 0.24）（P=0.040）。结论 过表达SHP-1基因抑制K562细胞增殖和集落形成，促进细胞凋亡，导致细胞周期阻滞在G0/G1期，并降低K562细胞中BCR-ABL1蛋白水平
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