%0 Journal Article
%T 过表达SHP-1对人慢性髓系白血病急变细胞系K562生物学特征的影响
%A 刘学东
%A 刘晓
%A 张永晓
%A 李志赏
%A 李英华
%A 罗建民
%A 郭秀芬
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.10.008
%X 摘要 目的 探讨SHP-1基因对K562细胞系增殖、凋亡、细胞周期分布和集落形成的影响。方法 将携带SHP-1基因和绿色荧光蛋白（EGFP）基因的慢病毒表达载体转染K562细胞，SYBR Green荧光定量PCR和Western blot法检测转染细胞SHP-1 mRNA和蛋白的表达情况；CCK-8法检测细胞增殖；流式细胞术检测细胞凋亡率和细胞周期分布；光学显微镜和电子显微镜下观察细胞形态；TUNEL法检测细胞凋亡；培养细胞集落并比较不同组间集落形成的数量和大小。结果 培养第3天，K562SHP-1细胞的OD值为（0.35±0.02），K562EGFP细胞的OD值为（0.47±0.05），两者差异有统计学意义（P=0.011）。随着培养天数的延长，与K562EGFP细胞相比K562SHP-1细胞出现G0/G1期细胞比例增加和G2/M+S期细胞比例降低及凋亡增加，培养第5天K562SHP-1细胞G0/G1期比例为（56.37±2.27）、凋亡率为（26.13±1.16），K562EGFP细胞G0/G1期比例为（31.67±3.21），凋亡率为（9.00±1.22），两者比较差有统计学意义（P=0.000）。K562SHP-1细胞集落形成能力（26.3±5.2）较K562EGFP细胞（54.7±8.6）有所降低（P=0.000）；K562SHP-1细胞BCR-ABL1蛋白的表达水平（0.78±0.15）低于K562EGFP细胞（1.27± 0.24）（P=0.040）。结论 过表达SHP-1基因抑制K562细胞增殖和集落形成，促进细胞凋亡，导致细胞周期阻滞在G0/G1期，并降低K562细胞中BCR-ABL1蛋白水平
%K SHP-1基因
%K K562细胞
%K 凋亡
%K 增殖
%K 细胞周期
%K 集落形成
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%U http://www.zlfzyj.com/CN/abstract/abstract8607.shtml