叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的机制
DOI: 10.3971/j.issn.1000-8578.2018.17.1303
Keywords: A Retrospective Study,Application of PSMA PET/CT Specific Molecular Imaging in Diagnosis of Non-prostate Cancer,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,A Comprehensive Review of Androgen Deprivation Therapy and Immunotherapy on Prostate Cancer,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Antitumor and Apoptosis Induction Effects of Gold Nanorods-mediated Near-infrared Photothermal Therapy on Gastric Cancer SGC-7901 Cells in vitro,Effects of MicroRNA-4465 on Apoptosis and Invasion of Colon Cancer Cells,Application of Superb Micro-vascular Imaging Technology in Guiding Accurately Targeted Biopsy of Prostate Cancer,Efficacy and Prognostic Factors of Docetaxel Rechallenge on Metastatic Castrationresistant Prostate Cancer Patients,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3,Regulatory Effect of Astragaloside A on Proliferation and Apoptosis of Human Colon Cancer SW480 Cell Line,Effects of Pachymaran on Proliferation, Migration and Pro-apoptosis of Human Cervical Carcinoma HeLa Cells and Its Mechanism
Abstract:
摘要 目的 探究叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的作用机制，为前列腺癌预防及治疗提供新的理论依据。方法 不同浓度叶黄素作用于PC3细胞，CCK8法检测细胞增殖情况；流式细胞仪检测细胞周期分布和凋亡变化；细胞划痕和Transwell实验观察细胞迁移和侵袭能力；RT-PCR和Western blot技术检测细胞中Bax、Bcl-2的mRNA水平和蛋白表达。结果 叶黄素显著抑制PC3细胞的增殖，并呈时间和浓度依赖性。叶黄素可以将细胞生长阻滞在G0/G1期，抑制细胞迁移和侵袭；还可促进细胞凋亡，使Bcl-2表达下降，Bax表达上升。叶黄素可在转录水平上下调Bcl-2 mRNA和上调BaxmRNA。结论 叶黄素抑制PC3细胞增殖并促进其凋亡，其机制可能与阻滞细胞周期、抑制细胞迁移、侵袭以及调节凋亡相关基因和蛋白表达有关
Full-Text
