%0 Journal Article
%T 叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的机制
%A 叶淑柯
%A 付蕾
%A 刘卫华
%A 王丽平
%A 王亚乐
%A 王明臣
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1303
%X 摘要 目的 探究叶黄素对人前列腺癌PC3细胞增殖和凋亡影响的作用机制，为前列腺癌预防及治疗提供新的理论依据。方法 不同浓度叶黄素作用于PC3细胞，CCK8法检测细胞增殖情况；流式细胞仪检测细胞周期分布和凋亡变化；细胞划痕和Transwell实验观察细胞迁移和侵袭能力；RT-PCR和Western blot技术检测细胞中Bax、Bcl-2的mRNA水平和蛋白表达。结果 叶黄素显著抑制PC3细胞的增殖，并呈时间和浓度依赖性。叶黄素可以将细胞生长阻滞在G0/G1期，抑制细胞迁移和侵袭；还可促进细胞凋亡，使Bcl-2表达下降，Bax表达上升。叶黄素可在转录水平上下调Bcl-2 mRNA和上调BaxmRNA。结论 叶黄素抑制PC3细胞增殖并促进其凋亡，其机制可能与阻滞细胞周期、抑制细胞迁移、侵袭以及调节凋亡相关基因和蛋白表达有关
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%U http://www.zlfzyj.com/CN/abstract/abstract9148.shtml