IL-6诱导小鼠骨髓中性粒细胞中MMP-9的生成与释放的实验
DOI: 10.3971/j.issn.1000-8578.2016.02.007
Keywords: 白细胞介素-6,信号转导及转录激活蛋白3,中性粒细胞,基质金属蛋白酶-9（MMP-9）,miR-216b Inhibits Cervical Cancer Cells Autophagy by Regulating Beclin-1 Expression,Interstitial Brachytherapy of Cervical Cancer,Effect of CGB5 on Human Epithelial Ovarian Cancer Cell Line OVCAR-3 in Vasculogenic Mimicry Formation,Advance of Prognostic Markers of Cervical Cancer,Relationship of Expression Difference of miR-658 and miR-492 with Pelvic Lymph Node Metastasis from Squamous Carcinoma of Cervix,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Effects of Progesterone on Expressions of p57kip2 and Cyclin E in Human Endometrioid Carcinoma Cells JEC,Inhibitory Effect of Thermosensitive Liposomes Combined with Microwave Thermotherapy on Human Cervical Carcinoma Cells HeLa,Bioinformatics Analysis of Genes Related to Multidrug Resistance in Ovarian Cancer,Expression and Significance of TNF-α and TNFR1 in Human Cervical Squamous Cell Carcinoma Tissues,Characteristics of CD44 and CD24-marked Cervical Cancer Cells Subgroups,Risk Factors Associated with Hematologic Toxicity in Concurrent Chemoradiotherapy and IMRT for Cervical Cancer,Clinical Investigation on Cytoreductive Surgery plus Hyperthermic Intraperitoneal Chemotherapy on Patients with Peritoneal Carcinomatosis from Epithelial Ovarian Cancer,Estradiol Activates MAPK Signaling Pathway and Its Effects on Proliferation of Endometrial Cancer Ishikawa Cells,A Prospective Clinical Trial of Treatment Models on Malignant Ovarian Germ Cell Tumor Patients
Abstract:
摘要 目的 探讨IL-6诱导小鼠骨髓中性粒细胞（PMN）生成和释放基质金属蛋白酶-9（MMP-9）的作用及激活途径。方法 分离小鼠骨髓PMN，将其分为空白对照组、IL-6刺激组、IL-6+DMSO组、IL-6+STAT3抑制剂Ⅷ组。实时荧光定量聚合酶链式反应检测细胞内MMP-9 mRNA表达，明胶酶谱检测PMN释放的MMP-9活性，Western blot检测PMN中STAT3蛋白磷酸化改变。结果 IL-6刺激后，PMN中MMP-9 mRNA水平明显升高（P=0.0050），明胶酶谱检测的释放到胞外MMP-9活性明显高于对照组（P=0.0087），IL-6刺激后PMN中STAT3磷酸化水平明显升高（P=0.0089）。在IL-6作用时加入特异性STAT3抑制剂Ⅷ后，MMP-9 mRNA水平、培养上清液中MMP-9活性以及PMN中磷酸化STAT3蛋白水平与IL-6刺激组相比均明显下降（P=0.0067, 0.048和0.0098）。结论 IL-6刺激骨髓PMN中MMP-9的生成和释放，该作用可能是通过诱导STAT3信号通路磷酸化实现的
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