%0 Journal Article
%T IL-6诱导小鼠骨髓中性粒细胞中MMP-9的生成与释放的实验
%A 刘颖华
%A 吴绪峰
%A 彭秋子
%A 袁冬妮
%A 颜彬
%A 高晗
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.02.007
%X 摘要 目的 探讨IL-6诱导小鼠骨髓中性粒细胞（PMN）生成和释放基质金属蛋白酶-9（MMP-9）的作用及激活途径。方法 分离小鼠骨髓PMN，将其分为空白对照组、IL-6刺激组、IL-6+DMSO组、IL-6+STAT3抑制剂Ⅷ组。实时荧光定量聚合酶链式反应检测细胞内MMP-9 mRNA表达，明胶酶谱检测PMN释放的MMP-9活性，Western blot检测PMN中STAT3蛋白磷酸化改变。结果 IL-6刺激后，PMN中MMP-9 mRNA水平明显升高（P=0.0050），明胶酶谱检测的释放到胞外MMP-9活性明显高于对照组（P=0.0087），IL-6刺激后PMN中STAT3磷酸化水平明显升高（P=0.0089）。在IL-6作用时加入特异性STAT3抑制剂Ⅷ后，MMP-9 mRNA水平、培养上清液中MMP-9活性以及PMN中磷酸化STAT3蛋白水平与IL-6刺激组相比均明显下降（P=0.0067, 0.048和0.0098）。结论 IL-6刺激骨髓PMN中MMP-9的生成和释放，该作用可能是通过诱导STAT3信号通路磷酸化实现的
%K 白细胞介素-6
%K 信号转导及转录激活蛋白3
%K 中性粒细胞
%K 基质金属蛋白酶-9（MMP-9）
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%U http://www.zlfzyj.com/CN/abstract/abstract8697.shtml