GM-CSF在替莫唑胺抗高级别胶质瘤中的作用
DOI: 10.3971/j.issn.1000-8578.2016.02.002
Keywords: 粒- 巨噬细胞集落刺激因 子,高级别胶质瘤细胞,六氧甲基鸟嘌呤DNA甲基转移酶,细胞周期,致死作用,Progress of Relationships of Calprotectin with Tumours,Effects of Gefitinib Combined with Bruceolic Oil Emulsion on Human Lung Adenocarcinoma Cell Lines and Its Mechanism,Effects of SHP-1 Overexpression on Biological Characteristics of K562 Cells,Effect of Silencing Toll-like Receptor 4 Expression on Cell Cycle and Apoptosis of Human Lung Adenocarcinoma Cells A549,Tumor Suppressor Roles of miR-100 in Esophageal Squamous Cell Carcinoma Cell Line Ec-109,Cisplatin-induced Senescence and Senescence-related Gene Expression Profiles in#br# Human Nasopharyngeal Carcinoma Cells CNE2,Role of IL-1RAP in Gliomas Cells and Its Relationship with STAT3,Effects of MNNG on Proliferation, Cycle and Apoptosis of Kazakh Normal Esophageal Epithelial Cells,Sequence-dependent Effects and Mechanism of Pemetrexed and Gefitinib on Human Lung Adenocarcinoma Cells,Effect of Ammonium Molybdate on Cell Proliferation and Cycle of Hepatoma and Colon Cancer,Tivozanib Inhibits Proliferation of Thyroid Cancer Cells and Vascular Endothelial Cells,Lithium Chloride Inhibits Proliferation of THP-1 Leukemia Cells and Activates Wnt Signaling Pathway,Molecular Cloning and Function Analysis of Human Cyclin D3 Gene Promoter Region#br# in A2780 Cells,Silence of BRG1 Gene Regulates Proliferation of Human Glioma Cell,Effects of Arenaria Kansuensis Aqueous Extract on Proliferation and Cell Cycle of Human Gastric Cancer Cell Line MGC-803
Abstract:
摘要 目的 探讨粒-巨噬细胞集落刺激因子（granulocyte-macrophage colony-stimulating factor, GMCSF）在替莫唑胺（temozolomide, TMZ）抗高级别胶质瘤中的作用及机制。方法 选取6例高级别胶质瘤患者来源的肿瘤组织培养肿瘤细胞，待细胞状态稳定后采用MTT法进行细胞增殖毒性实验，流式细胞仪检测细胞周期变化和细胞凋亡情况，甲基化特异性PCR和免疫组织化学染色法分别检测六氧甲基鸟嘌呤DNA甲基转移酶（O6-methylguanine-DNA methyltransferase, MGMT）基因启动子甲基化状态和MGMT蛋白表达水平。 结果 MTT实验显示，GM-CSF处理组的细胞存活率与对照组相比均有不同程度的增加。MGMT基因启动子甲基化的3例细胞，GM-CSF+TMZ组的细胞存活率比TMZ组显著降低（P<0.05），另3例MGMT启动子非甲基化细胞，GM-CSF+TMZ组与TMZ组相比，其存活率差异均无统计学意义（P>0.05）。6例细胞GM-CSF组的G1期细胞比例均比对照组降低，而S期细胞比例GMCSF处理组较对照组显著增加（P<0.05）。流式细胞仪凋亡检测显示，MGMT启动子甲基化的3例细胞，GM-CSF+TMZ组凋亡率与单药TMZ组凋亡率相比均显著增加（P<0.05），而MGMT非甲基化细胞GM-CSF+TMZ组与单药TMZ组凋亡率相比无统计学差异。结论 GM-CSF可通过诱导高级别胶质瘤细胞快速进入细胞周期，显著提高TMZ对MGMT基因启动子甲基化的高级别胶质瘤细胞的杀伤作用，而对MGMT基因启动子非甲基化高级别胶质瘤细胞的作用不明显
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