%0 Journal Article
%T GM-CSF在替莫唑胺抗高级别胶质瘤中的作用
%A 屈鸣麒
%A 张慧
%A 杨冬谊
%A 步星耀
%A 闫兆月
%A 马春晓
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.02.002
%X 摘要 目的 探讨粒-巨噬细胞集落刺激因子（granulocyte-macrophage colony-stimulating factor, GMCSF）在替莫唑胺（temozolomide, TMZ）抗高级别胶质瘤中的作用及机制。方法 选取6例高级别胶质瘤患者来源的肿瘤组织培养肿瘤细胞，待细胞状态稳定后采用MTT法进行细胞增殖毒性实验，流式细胞仪检测细胞周期变化和细胞凋亡情况，甲基化特异性PCR和免疫组织化学染色法分别检测六氧甲基鸟嘌呤DNA甲基转移酶（O6-methylguanine-DNA methyltransferase, MGMT）基因启动子甲基化状态和MGMT蛋白表达水平。 结果 MTT实验显示，GM-CSF处理组的细胞存活率与对照组相比均有不同程度的增加。MGMT基因启动子甲基化的3例细胞，GM-CSF+TMZ组的细胞存活率比TMZ组显著降低（P<0.05），另3例MGMT启动子非甲基化细胞，GM-CSF+TMZ组与TMZ组相比，其存活率差异均无统计学意义（P>0.05）。6例细胞GM-CSF组的G1期细胞比例均比对照组降低，而S期细胞比例GMCSF处理组较对照组显著增加（P<0.05）。流式细胞仪凋亡检测显示，MGMT启动子甲基化的3例细胞，GM-CSF+TMZ组凋亡率与单药TMZ组凋亡率相比均显著增加（P<0.05），而MGMT非甲基化细胞GM-CSF+TMZ组与单药TMZ组凋亡率相比无统计学差异。结论 GM-CSF可通过诱导高级别胶质瘤细胞快速进入细胞周期，显著提高TMZ对MGMT基因启动子甲基化的高级别胶质瘤细胞的杀伤作用，而对MGMT基因启动子非甲基化高级别胶质瘤细胞的作用不明显
%K 粒- 巨噬细胞集落刺激因 子
%K 高级别胶质瘤细胞
%K 六氧甲基鸟嘌呤DNA甲基转移酶
%K 细胞周期
%K 致死作用
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%U http://www.zlfzyj.com/CN/abstract/abstract8692.shtml