缺氧对喉癌Hep-2细胞HIF-1α、GLUT-1、MMP-2表达的影响
DOI: 10.3971/j.issn.1000-8578.2016.08.003
Keywords: 喉癌,缺氧,缺氧诱导因子-1&alpha,葡萄糖转运蛋白1型,基质金属蛋白酶-2,Correlation Between Expression of SIX1, TGF-β and VEGF-C in Primarily Cultured Human Laryngeal Squamous Cell Carcinoma Cells,In vitro Investigation of Antitumor Efficacy of DpC on Head and Neck Cancer,Surgical Operation and Perioperative Management for Elderly Hypopharyngeal Cancer Patients,Relationship between Local Recurrence and Anterior Commissure Involvement in Early Glottic Laryngocarcinoma after Radiofrequency Coblation,Clinical Efficacy and Prognosis of Concomitant Chemo-radiotherapy and Simple Radiotherapy on Patients with Advanced Laryngeal Cancer after Laryngectomy,Association between Brg1 Single Nucleotide Polymorphism and Laryngo Carcinoma,Anti-laryngocarcinoma Immunity of DC Vaccine which Silenced socs1 by siRNA,Expression and Significance of EGFR and TSLC1 in Different Laryngeal Lesions,Influence of Anti-apoptosis Induced by Recombinant survivin Adenovirus in Laryngeal Cacinoma Cells,Effects of Nimesulide on Expression of CD44 and MMP-7 in Nude Mice Transplanted Hep-2 Cell Line of Human Laryngeal Squamous Cell Carcinoma,Expression of MUC18 and Its Correlation with Counts of Microvessel and Lymph Vessel in Laryngeal Squamous Cell Carcinoma Tissues,Three Human Carcinoma Cell Lines with Different Sensitivity to Adenovirus Expressed E1A Gene,Effect of Simultaneously Blocking VEGF,hTERT and Bcl-xl Expression on Laryngeal Squamous Carcinoma
Abstract:
摘要 目的 探讨缺氧对喉癌Hep-2细胞增殖、迁移和侵袭以及缺氧诱导因子-1α（HIF-1α）、葡萄糖转运蛋白-1（GLUT-1）和基质金属蛋白酶-2（MMP-2）表达的影响。方法 MTT法检测缺氧不同时间点细胞增殖状况；Transwell和划痕实验分别检测缺氧对喉癌Hep-2细胞侵袭和迁移能力的影响；Western blot和实时定量反转录聚合酶链反应（RT-PCR）检测缺氧后不同时间点HIF-1α、GLUT-1、MMP-2蛋白及mRNA表达的情况。结果 在缺氧条件下（1％O2、5％CO2和94％N2），Hep-2细胞的存活率随缺氧时间的延长逐渐升高，迁移和侵袭能力增强；随缺氧时间延长，Hep-2细胞HIF-1α蛋白表达水平明显增加，mRNA水平变化不明显；随缺氧时间延长，GLUT-1、MMP-2的mRNA水平和蛋白水平均明显增加。 结论 缺氧环境下，喉癌肿瘤细胞通过上调HIF-1α、GLUT-1、MMP-2的表达，强化其增殖、侵袭、转移的能力，促进喉癌的发展
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