%0 Journal Article
%T 缺氧对喉癌Hep-2细胞HIF-1α、GLUT-1、MMP-2表达的影响
%A 廖敏
%A 张云桂
%A 邓碧凡
%A 邱荣敏
%A 马燕
%A 高波
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.08.003
%X 摘要 目的 探讨缺氧对喉癌Hep-2细胞增殖、迁移和侵袭以及缺氧诱导因子-1α（HIF-1α）、葡萄糖转运蛋白-1（GLUT-1）和基质金属蛋白酶-2（MMP-2）表达的影响。方法 MTT法检测缺氧不同时间点细胞增殖状况；Transwell和划痕实验分别检测缺氧对喉癌Hep-2细胞侵袭和迁移能力的影响；Western blot和实时定量反转录聚合酶链反应（RT-PCR）检测缺氧后不同时间点HIF-1α、GLUT-1、MMP-2蛋白及mRNA表达的情况。结果 在缺氧条件下（1％O2、5％CO2和94％N2），Hep-2细胞的存活率随缺氧时间的延长逐渐升高，迁移和侵袭能力增强；随缺氧时间延长，Hep-2细胞HIF-1α蛋白表达水平明显增加，mRNA水平变化不明显；随缺氧时间延长，GLUT-1、MMP-2的mRNA水平和蛋白水平均明显增加。 结论 缺氧环境下，喉癌肿瘤细胞通过上调HIF-1α、GLUT-1、MMP-2的表达，强化其增殖、侵袭、转移的能力，促进喉癌的发展
%K 喉癌
%K 缺氧
%K 缺氧诱导因子-1&alpha
%K 葡萄糖转运蛋白1型
%K 基质金属蛋白酶-2
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%K Expression of MUC18 and Its Correlation with Counts of Microvessel and Lymph Vessel in Laryngeal Squamous Cell Carcinoma Tissues
%K Three Human Carcinoma Cell Lines with Different Sensitivity to Adenovirus Expressed E1A Gene
%K Effect of Simultaneously Blocking VEGF
%K hTERT and Bcl-xl Expression on Laryngeal Squamous Carcinoma
%U http://www.zlfzyj.com/CN/abstract/abstract8809.shtml