RNA干扰沉默MSI1表达对人脑胶质瘤细胞化疗敏感度的影响
DOI: 10.3971/j.issn.1000-8578.2016.09.005
Keywords: MSI1,RNA干扰,胶质瘤,药物敏感度,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Effect of Gene Silencing of RNF2 on Radiosensitivity of Esophageal Carcinoma Cells,MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells,Effect of siRNA-mediated notch1 Gene Silencing on Apoptosis and Radiosensitivity of Glioma Cell Line U87-EGFRvⅢ,In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG,Review on Radiation Dose Fractionation on High-grade Glioma Patients,LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma,GM-CSF Enhances Effect of TMZ on High-grade Glioma Cells,Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells,Relationship Between FoxQ1 and Epidermal Growth Factor Receptor Gene Expression in Colorectal Cancer,Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion,Construction and Screening of shRNA Expression Plasmids Targeting at cortactin Gene,Research Status and Development of Adherens Junction Associated Protein-1 in Gliomas,Effects of Vascular Endothelial Cells on Invasion of Glioma Cells via Notch Signal Pathway,Down-regulating UHRF1 Expression could Inhibit Progression of Hepatocellular Carcinoma
Abstract:
摘要 目的 探讨RNA干扰沉默MSI1表达对人脑胶质瘤细胞化疗药物敏感度的影响。方法 构建针对MSI1 mRNA的小分子RNA干扰（siRNA）重组质粒pSIREN-MSI1，转染人脑胶质瘤U-87MG细胞；Western blot检测U-87MG细胞内MSI1蛋白的表达；CCK-8法观察U-87MG细胞对化疗药物替莫唑胺（TMZ）敏感度的改变；流式细胞仪（FCM）检测pSIREN-MSI1转染后对U-87MG细胞凋亡的影响。结果 成功构建了重组质粒pSIREN-MSI1，并成功转染U-87MG细胞；Western blot结果显示pSIREN-MSI1抑制U-87MG细胞中MSI1蛋白的表达（P<0.01）；CCK-8结果显示在替莫唑胺作用下，pSIREN-MSI1组U-87MG细胞的存活率明显下降（P<0.01）；FCM结果表明pSIREN-MSI1转染后明显提高U-87MG细胞的凋亡率（P<0.01）。结论 MSI1 siRNA能特异性抑制人脑胶质瘤细胞U-87MG细胞中MSI1的表达，增强人脑胶质瘤U-87MG细胞对化疗药物的敏感度，并促进细胞的凋亡
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