%0 Journal Article
%T RNA干扰沉默MSI1表达对人脑胶质瘤细胞化疗敏感度的影响
%A 付锴
%A 刘细国
%A 宫睿
%A 江普查
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.09.005
%X 摘要 目的 探讨RNA干扰沉默MSI1表达对人脑胶质瘤细胞化疗药物敏感度的影响。方法 构建针对MSI1 mRNA的小分子RNA干扰（siRNA）重组质粒pSIREN-MSI1，转染人脑胶质瘤U-87MG细胞；Western blot检测U-87MG细胞内MSI1蛋白的表达；CCK-8法观察U-87MG细胞对化疗药物替莫唑胺（TMZ）敏感度的改变；流式细胞仪（FCM）检测pSIREN-MSI1转染后对U-87MG细胞凋亡的影响。结果 成功构建了重组质粒pSIREN-MSI1，并成功转染U-87MG细胞；Western blot结果显示pSIREN-MSI1抑制U-87MG细胞中MSI1蛋白的表达（P<0.01）；CCK-8结果显示在替莫唑胺作用下，pSIREN-MSI1组U-87MG细胞的存活率明显下降（P<0.01）；FCM结果表明pSIREN-MSI1转染后明显提高U-87MG细胞的凋亡率（P<0.01）。结论 MSI1 siRNA能特异性抑制人脑胶质瘤细胞U-87MG细胞中MSI1的表达，增强人脑胶质瘤U-87MG细胞对化疗药物的敏感度，并促进细胞的凋亡
%K MSI1
%K RNA干扰
%K 胶质瘤
%K 药物敏感度
%K Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells
%K Effect of Gene Silencing of RNF2 on Radiosensitivity of Esophageal Carcinoma Cells
%K MDM2 siRNA Inhibits Angiogenesis by Reducing Vascular Endothelial Growth Factor Production in Breast Cancer Cells
%K Effect of siRNA-mediated notch1 Gene Silencing on Apoptosis and Radiosensitivity of Glioma Cell Line U87-EGFRvⅢ
%K In vitro Investigation on Effect of Lentiviral Vector-mediated RNA Interference Inhibiting VASH1 Expression on Proliferation and Apoptosis of Human Glioma Cells U-87MG
%K Review on Radiation Dose Fractionation on High-grade Glioma Patients
%K LncRNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma
%K GM-CSF Enhances Effect of TMZ on High-grade Glioma Cells
%K Effects for Histone Deacetylase Inhibitors on Proliferation and MKK7 Expression in Glioma Cells
%K Relationship Between FoxQ1 and Epidermal Growth Factor Receptor Gene Expression in Colorectal Cancer
%K Effect of HSP70 and iNOS in Process of Hyperthermia Reducing Glioma Invasion
%K Construction and Screening of shRNA Expression Plasmids Targeting at cortactin Gene
%K Research Status and Development of Adherens Junction Associated Protein-1 in Gliomas
%K Effects of Vascular Endothelial Cells on Invasion of Glioma Cells via Notch Signal Pathway
%K Down-regulating UHRF1 Expression could Inhibit Progression of Hepatocellular Carcinoma
%U http://www.zlfzyj.com/CN/abstract/abstract8828.shtml