胃黏膜瘤变过程中差异蛋白的表达
DOI: 10.3971/j.issn.1000-8578.2018.17.1439
Keywords: 胃癌,异型增生,分子标志物,蛋白质组学,Mechanism of miRNA-451 Regulating Resistance of Gastric Cancer Cells to 5-Fu Through MRP,Effect of Da Vinci Robot Radical Gastrectomy on Gastrointestinal Motility and Gastrointestinal Hormones of Gastric Cancer Patients,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Related Factors and Risk Model Prediction of Pneumonia After ESD in Patients with Early Gastric Cancer,Effect of FBXL20 on Proliferation, Migration and Invasion of Gastric Cancer Cells,A Multi-center Randomized Controlled Clinical Study of Kang’ai Injection Combined with OFL Regimen on Gastric Cancer Patients,Expression and Clinical Significance of miR-181d in Serum and PDCD4 in Gastric Cancer Patients,Expression of CRT and CD47 in Gastric Carcinoma and Their Significance,Expression of KIF3A in Different Gastric Mucosal Lesions, Metastatic Lymph Nodes and Its Relationship with Metastasis and Prognosis of Gastric Adenocarcinoma,Effect of Yes-associated Protein Knockdown on Apoptosis of Gastric Cancer BGC823 Cells Through Wnt/β-catenin Signaling Pathway,Short-term Efficacy and Safety of Total Laparoscopic and Laparoscopic-assisted Radical Gastrectomy on Distal Gastric Cancer,Annexin A5 Knockdown Prompts Proliferation of Gastric Cancer MKN-45 Cells,Application of Carbon Nano Label in Delta Anastomosis of Total Laparoscopic Radical Gastrectomy for Distal Gastric Cancer,Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT,Clinical Value of Abnormal Prothrombin Combined with CEA, CA72-4 and CA199 on Patients with Gastric Cancer
Abstract:
摘要 目的 探讨胃癌发生发展的不同阶段（正常胃黏膜组织、反应性增生、低级别上皮内瘤变、高级别上皮内瘤变及黏膜内腺癌）差异蛋白的表达。方法 以胃窦部肠型胃癌为研究对象，选取正常、反应性增生、低/高级别上皮内瘤变、黏膜内癌5组各30例共150例组织标本，用非标记蛋白质组学（labelfree）联合液性色谱质谱（LC-MC/MC）筛选差异蛋白，并评估差异蛋白潜在的生物学功能。结果 筛选出了8个差异蛋白，和正常黏膜相比，在低级别上皮内瘤变—高级别上皮内瘤变及黏膜内癌的发展过程中，LAMN1、ERGIC1、UQCRFS1、UQCRH和CISD1这5个蛋白质的表达量逐渐降低，表达量逐渐升高的蛋白质有HMGB1、DNA-PKcs和14-3-3。结论 在胃癌发生发展的不同阶段，蛋白质的表达存在明显的差异，这些差异蛋白具有成为胃癌早期诊断及靶向分子治疗标志物的潜能
Full-Text
