LSD1调控Foxo3a对卵巢癌细胞增殖和迁移的影响
DOI: 10.3971/j.issn.1000-8578.2018.17.1548
Keywords: A Meta-analysis,Efficacy of 93 Cases of Recurrent Ovarian Cancer,Meta-analysis of Efficacy and Safety of Jinlong Capsule Combined with Chemoradiotherapy on Liver Metastases,Expression and Functional of LncRNA XIST in MCF-7 Breast Cancer Cells,Autophagy Related Protein 5 Regulates Colon Cancer Cell Senescence Through p53/p21 Pathway,Adverse Reaction of HIPEC Combined with Intravenous Chemotherapy on Ovarian Cancer Patients,Clinicopathologic Characteristics and Prognosis of De Novo Metastatic Breast Cancer Patients,Advances in Role of Cyclooxygenase 2 in Development and Progression of Cancer,Bone Metastasis in Children with Metanephric Adenosarcoma: A Case Report and Literature Review#br#,Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells,Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells,Risk Factors of Pathological Upstaging for Patients with Clinical T2N0M0 Esophageal Squamous Cell Carcinoma,Efficacy and Prognostic Factors of Docetaxel Rechallenge on Metastatic Castrationresistant Prostate Cancer Patients,Advances in Molecular Mechanisms of Early Bone Metastasis,Mechanism of D-bifunctional Protein Promoting Formation of Hepatocellular Carcinoma in Rat via STAT3
Abstract:
摘要 目的 探讨赖氨酸特异性去甲基化酶1（LSD1）如何通过调控转录因子Foxo3a影响卵巢癌细胞增殖和迁移。方法 实验组A：取诱导型稳定干扰LSD1表达的人卵巢癌HO8910细胞株（HO8910-LSD1-shRNA）分为观察组和对照组，蛋白质印迹法检测LSD1和Foxo3a蛋白表达水平；实验组B：将HO8910-LSD1-shRNA细胞分为对照组、Dox组、A6730组和联合组，CCK-8检测各组细胞增殖抑制率，Transwell小室检测各组细胞迁移能力，蛋白质印迹法检测EMT相关蛋白表达。结果 在实验组A中，观察组LSD1蛋白水平随Dox浓度增加逐渐下降，而Foxo3a蛋白表达水平逐渐升高。在实验组B中，与对照组比较，Dox组、A6730组和联合组细胞增殖抑制率、细胞迁移率均显著减少（均P<0.05）；联合组较Dox组，细胞增殖抑制率、细胞迁移率均显著减少（均P<0.05）。与对照组比较，Dox组、A6730组和联合组E-cadherin蛋白表达水平明显升高，而N-cadherin和Snail蛋白水平降低。与Dox组和A6730组比较，联合组E-cadherin表达量增加，而N-cadherin及Snail表达量减少。结论 敲低LSD1基因表达可以上调转录因子Foxo3a蛋白水平，从而抑制卵巢癌HO8910细胞增殖和转移
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