%0 Journal Article
%T LSD1调控Foxo3a对卵巢癌细胞增殖和迁移的影响
%A 吴朝阳
%A 周昱霖
%A 王品昊
%A 钱尧
%A 魏野
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.17.1548
%X 摘要 目的 探讨赖氨酸特异性去甲基化酶1（LSD1）如何通过调控转录因子Foxo3a影响卵巢癌细胞增殖和迁移。方法 实验组A：取诱导型稳定干扰LSD1表达的人卵巢癌HO8910细胞株（HO8910-LSD1-shRNA）分为观察组和对照组，蛋白质印迹法检测LSD1和Foxo3a蛋白表达水平；实验组B：将HO8910-LSD1-shRNA细胞分为对照组、Dox组、A6730组和联合组，CCK-8检测各组细胞增殖抑制率，Transwell小室检测各组细胞迁移能力，蛋白质印迹法检测EMT相关蛋白表达。结果 在实验组A中，观察组LSD1蛋白水平随Dox浓度增加逐渐下降，而Foxo3a蛋白表达水平逐渐升高。在实验组B中，与对照组比较，Dox组、A6730组和联合组细胞增殖抑制率、细胞迁移率均显著减少（均P<0.05）；联合组较Dox组，细胞增殖抑制率、细胞迁移率均显著减少（均P<0.05）。与对照组比较，Dox组、A6730组和联合组E-cadherin蛋白表达水平明显升高，而N-cadherin和Snail蛋白水平降低。与Dox组和A6730组比较，联合组E-cadherin表达量增加，而N-cadherin及Snail表达量减少。结论 敲低LSD1基因表达可以上调转录因子Foxo3a蛋白水平，从而抑制卵巢癌HO8910细胞增殖和转移
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%U http://www.zlfzyj.com/CN/abstract/abstract9207.shtml