胶质瘤细胞中IL-1RAP的作用及其与STAT3的关系
DOI: 10.3971/j.issn.1000-8578.2015.01.006
Keywords: 白介素1受体辅助蛋白,转录活化因子3,胶质瘤,细胞周期,凋亡,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of RNA Interference Decreasing MSI1 Expression on Chemosensitivity of Human Glioma Cells,Effect of GANT61 on Apoptosis of Esophageal Squamous Carcinoma Cells and Its Mechanism,Progress of Relationships of Calprotectin with Tumours,Experimental Investigation on Apoptosis of Cervical Cancer Cells Promoted by NF-κB Decoy ODN,Cryptotanshinone Promotes Apoptosis of A549 Cells via Inhibiting Cap-dependent mRNA Translation,Effect of Terpinen-4-ol on Proliferation and Apoptosis of Human Multiple Myeloma Cells U266,Periplocin Extracted from Cortex Periplocae(CPP) Induced Apoptosis of Gastric Cancer Cells MGC-803 Through Lysosomal Pathway,Research Advances of ASPP in Regulating Apoptosis of Tumor Cells,Effect of siRNA-mediated notch1 Gene Silencing on Apoptosis and Radiosensitivity of Glioma Cell Line U87-EGFRvⅢ,Effect of AT Motif Binding Factor 1 on Proliferation and Invasion of Colorectal Carcinoma Cell Line LOVO
Abstract:
摘要 目的 研究白介素1受体辅助蛋白（IL-1RAP）在胶质瘤中的作用及其与转录活化因子3（STAT3）的关系。方法 构建IL-1RAP表达载体，转染胶质瘤细胞系U251，利用流式±细胞仪检测转染IL-1RAP对U251细胞周期及凋亡影响。利用免疫共沉淀钓取STAT3，检测转染IL-1RAP后STAT3的表达情况，并利用免疫荧光方法检测IL-1RAP与STAT3的共定位情况。结果 IL-1RAP蛋白定位于胶质瘤细胞核。与转染空质粒对照组相比，IL-1RAP有明显的促进肿瘤细胞凋亡[(52.10±5.51)% vs.(7.57± 0.54)%, P＜0.05]和周期阻滞作用[(68.22±1.96)% vs.(38.31±7.22)%, P＜0.05]。通过免疫共沉淀和共定位证实IL-1RAP和STAT3可以相互作用。结论 IL-1RAP有抑制胶质瘤细胞增殖、促进细胞凋亡的作用。IL-1RAP可能通过与STAT3相互作用进入细胞核发挥促进细胞凋亡和抑制细胞周期的作用
Full-Text
