%0 Journal Article
%T 胶质瘤细胞中IL-1RAP的作用及其与STAT3的关系
%A 刘卫东
%A 宫剑
%A 王建功
%A 胡万宁
%A 邢树山
%A 郭智珍
%J 肿瘤防治研究
%D 2015
%R 10.3971/j.issn.1000-8578.2015.01.006
%X 摘要 目的 研究白介素1受体辅助蛋白（IL-1RAP）在胶质瘤中的作用及其与转录活化因子3（STAT3）的关系。方法 构建IL-1RAP表达载体，转染胶质瘤细胞系U251，利用流式±细胞仪检测转染IL-1RAP对U251细胞周期及凋亡影响。利用免疫共沉淀钓取STAT3，检测转染IL-1RAP后STAT3的表达情况，并利用免疫荧光方法检测IL-1RAP与STAT3的共定位情况。结果 IL-1RAP蛋白定位于胶质瘤细胞核。与转染空质粒对照组相比，IL-1RAP有明显的促进肿瘤细胞凋亡[(52.10±5.51)% vs.(7.57± 0.54)%, P＜0.05]和周期阻滞作用[(68.22±1.96)% vs.(38.31±7.22)%, P＜0.05]。通过免疫共沉淀和共定位证实IL-1RAP和STAT3可以相互作用。结论 IL-1RAP有抑制胶质瘤细胞增殖、促进细胞凋亡的作用。IL-1RAP可能通过与STAT3相互作用进入细胞核发挥促进细胞凋亡和抑制细胞周期的作用
%K 白介素1受体辅助蛋白
%K 转录活化因子3
%K 胶质瘤
%K 细胞周期
%K 凋亡
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%U http://www.zlfzyj.com/CN/abstract/abstract8388.shtml