沉默B7-H3基因表达对人肝癌细胞侵袭能力的影响
DOI: 10.3971/j.issn.1000-8578.2016.12.007
Keywords: A Meta-analysis,Effect of Down-regulation of MLLT3/AF9 Expression on Proliferation and Invasion Abilities of Hepatoma SMMC-7721 Cells,Expression of miR-155/PTEN Axis in Hepatocellular Carcinoma and Its Mechanism,Advances of CXCL12-CXCR4/CXCR7 Biological Axis in Hepatocellular Carcinoma,Effect of IL-4/IL-4R on Biological Behavior of Hepatocellular Carcinoma and Its Underlying Mechanism,Primary Hepatocellular Carcinoma with Isolated Sinonasal Metastasis: One Case Report and Literature Review,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,siRNA-mediated Inhibition of MTH1 Gene Expression Promotes Apoptosis and Reduces Migration of Hepatocellular Carcinoma HepG2 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Prognosis of HBV-related Hepatocellular Carcinoma Patients Treated with Nucleos(t)ide Analogue Therapy Combined with Transarterial Chemoembolization,Immune Killing Effect of Silencing TIPE2 Expression on T Lymphocytes Against Lung Adenocarcinoma Cells LA795,Correlation of ChREBP mRNA Expression and Its CpG Island Methylation in Human Hepatocellular Carcinoma
Abstract:
摘要 目的 研究靶向沉默B7-H3基因表达对HepG2细胞侵袭能力的影响及可能机制。方法 设计针对B7-H3基因的shRNA沉默质粒，转染HepG2细胞，下调B7-H3的表达。划痕修复实验检测转染前后HepG2细胞移行能力的变化，Transwell实验检测基因沉默对细胞侵袭能力的影响，MST-1法和ELISA凋亡试剂盒分别检测细胞增殖和凋亡水平变化。通过Western blot实验和明胶酶谱实验检测侵袭相关分子MMP-2、MMP-9的表达和活性变化。结果 成功构建B7-H3 shRNA沉默质粒并转染HepG2细胞。与对照质粒转染组和未转染组比较，沉默B7-H3基因表达后，B7-H3 shRNA转染组HepG2细胞的移行（24 h: P=0.001; 48 h: P<0.001; 72 h: P<0.001）和侵袭（P<0.001）能力受到显著抑制，细胞的增殖和凋亡水平没有明显变化（P>0.05）。MMP-2、MMP-9的蛋白表达（MMP-2: P<0.001; MMP-9: P=0.007）和活性（MMP-2: P<0.001; MMP-9: P<0.001）均显著下降。结论 通过B7-H3 shRNA沉默质粒靶向沉默B7-H3的基因表达能抑制HepG2细胞的侵袭能力，其机制可能与抑制MMP-2、MMP-9的表达和活性有关
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