%0 Journal Article
%T 沉默B7-H3基因表达对人肝癌细胞侵袭能力的影响
%A 孙殿兴
%A 康富标
%A 李东
%A 王燕
%A 王玲
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.12.007
%X 摘要 目的 研究靶向沉默B7-H3基因表达对HepG2细胞侵袭能力的影响及可能机制。方法 设计针对B7-H3基因的shRNA沉默质粒，转染HepG2细胞，下调B7-H3的表达。划痕修复实验检测转染前后HepG2细胞移行能力的变化，Transwell实验检测基因沉默对细胞侵袭能力的影响，MST-1法和ELISA凋亡试剂盒分别检测细胞增殖和凋亡水平变化。通过Western blot实验和明胶酶谱实验检测侵袭相关分子MMP-2、MMP-9的表达和活性变化。结果 成功构建B7-H3 shRNA沉默质粒并转染HepG2细胞。与对照质粒转染组和未转染组比较，沉默B7-H3基因表达后，B7-H3 shRNA转染组HepG2细胞的移行（24 h: P=0.001; 48 h: P<0.001; 72 h: P<0.001）和侵袭（P<0.001）能力受到显著抑制，细胞的增殖和凋亡水平没有明显变化（P>0.05）。MMP-2、MMP-9的蛋白表达（MMP-2: P<0.001; MMP-9: P=0.007）和活性（MMP-2: P<0.001; MMP-9: P<0.001）均显著下降。结论 通过B7-H3 shRNA沉默质粒靶向沉默B7-H3的基因表达能抑制HepG2细胞的侵袭能力，其机制可能与抑制MMP-2、MMP-9的表达和活性有关
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%U http://www.zlfzyj.com/CN/abstract/abstract8889.shtml