Garcinone E抑制鼻咽癌细胞增殖、迁移和侵袭能力的研究
DOI: 10.3971/j.issn.1000-8578.2018.18.0161
Keywords: Garcinone E,鼻咽癌细胞,上皮间质转化,Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells,Significance of E47 and LOXL2 Expressions in Esophageal Squamous Cell Carcinoma Tissues,Gab2 Promotes Invasion and Metastasis of Gastric Cancer via Regulating EMT,Regulatory Effects of miR-205 on Invasion and Migration of Gastric Cancer Cells and Potential Mechanism,Advances of Endoplasmic Reticulum Stress/Unfolded Protein Response Promote Tumor Invasion,5-azacytidine Increases Radiation Sensitivity of Nasopharyngeal Carcinoma Cell Line C666-1,Effect of Hypoxia on Epithelia-mesenchymal Transition of Prostate Cancer PC3 Cells,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Correlation Between Epithelial-mesenchymal Transition and Sensitivity to EGFR Targeted Monoclonal Antibody in Triple Negative Breast Cancer Cells,Effects of miR-711 on Invasion, Metastasis and Epithelial Mesenchymal Transition of Gastric Cancer Cells MGC-803,Role of Cancer-associated Fibroblasts in Occurrence and Development of Colorectal Cancer,Role of Hepatocyte Growth Factor Receptor c-met Regulating Epithelial-mesenchymal Transition in Metastasis of Colorectal Cancer,Nrf2-mediated Multidrug Resistance after Epithelial-mesenchymal Transition in Lung Cancer Cells,Effect of Transcription Factor Snail on Invasion and Migration of Esophageal Carcinoma Cell Line Eca-109,Progress in Relationship between Sox2 Gene and Cancer
Abstract:
摘要 目的 探讨源于岭南山竹的天然产物单体Garcinone E对鼻咽癌细胞增殖、迁移侵袭能力的影响及其分子机制。方法 MTS法检测鼻咽癌HONE1和HK1细胞的增殖率；Transwell细胞小室检测HONE1和HK1细胞迁移和侵袭的能力；Western blot和q-PCR法检测HONE1和HK1细胞上皮间充质转化（EMT）相关标志性蛋白和基因表达及可能的信号通路。结果 MTS 24 h结果显示Garcinone E可显著抑制鼻咽癌细胞HONE1和HK1的生长，并和浓度呈正相关作用。Transwell小室实验显示，与对照组相比，Garcinone E的给药浓度为7.5 μmol/L时，HONE1和HK1细胞穿过小室的数量明显减少，其迁移和侵袭能力明显受到抑制。Western blot检测发现Garcinone E呈剂量依赖性地下调p-Vimentin、Snail及Slug蛋白的表达，同时增加E-cadherin蛋白的表达。q-PCR表明Garcinone E呈剂量依赖性地上调两种鼻咽癌细胞中E-cadherin的mRNA表达。结论 Garcinone E能够有效抑制鼻咽癌细胞增殖并抑制鼻咽癌细胞迁移和侵袭能力，其机制可能与抑制鼻咽癌细胞EMT的发生有关
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