%0 Journal Article
%T Garcinone E抑制鼻咽癌细胞增殖、迁移和侵袭能力的研究
%A 刘夏
%A 卢奕
%A 夏玉对
%A 孔晓阳
%A 张健
%A 董彩华
%J 肿瘤防治研究
%D 2018
%R 10.3971/j.issn.1000-8578.2018.18.0161
%X 摘要 目的 探讨源于岭南山竹的天然产物单体Garcinone E对鼻咽癌细胞增殖、迁移侵袭能力的影响及其分子机制。方法 MTS法检测鼻咽癌HONE1和HK1细胞的增殖率；Transwell细胞小室检测HONE1和HK1细胞迁移和侵袭的能力；Western blot和q-PCR法检测HONE1和HK1细胞上皮间充质转化（EMT）相关标志性蛋白和基因表达及可能的信号通路。结果 MTS 24 h结果显示Garcinone E可显著抑制鼻咽癌细胞HONE1和HK1的生长，并和浓度呈正相关作用。Transwell小室实验显示，与对照组相比，Garcinone E的给药浓度为7.5 μmol/L时，HONE1和HK1细胞穿过小室的数量明显减少，其迁移和侵袭能力明显受到抑制。Western blot检测发现Garcinone E呈剂量依赖性地下调p-Vimentin、Snail及Slug蛋白的表达，同时增加E-cadherin蛋白的表达。q-PCR表明Garcinone E呈剂量依赖性地上调两种鼻咽癌细胞中E-cadherin的mRNA表达。结论 Garcinone E能够有效抑制鼻咽癌细胞增殖并抑制鼻咽癌细胞迁移和侵袭能力，其机制可能与抑制鼻咽癌细胞EMT的发生有关
%K Garcinone E
%K 鼻咽癌细胞
%K 上皮间质转化
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%U http://www.zlfzyj.com/CN/abstract/abstract9222.shtml