长链非编码RNA LOC100294362对乳腺癌细胞增殖和侵袭的影响
DOI: 10.3971/j.issn.1000-8578.2016.05.010
Keywords: 长链非编码RNA,LOC100294362,MCF-7细胞,增殖,侵袭,p53,Autophagic Cell Death Induced by GANT61 Inhibits Proliferation of Human Breast Cancer Cell Line MCF-7,Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells,Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88,Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells,Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway,Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells,Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism,Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism,Effect of RORα Overexpression on Proliferation, Migration and Invasion of Human Gastric MGC803 Cells,Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients,Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro,Effects on Cell Proliferation, Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway,Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism,Progress of TBX3 in Development of Malignant Tumors,Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism
Abstract:
摘要 目的 探讨长链非编码RNA LOC100294362在乳腺癌组织及细胞系中的表达情况，观察LOC100294362对乳腺癌MCF-7细胞增殖和侵袭的影响，并探索其可能的作用机制。方法 采用定量反转录PCR（qRT-PCR）方法检测乳腺癌组织及细胞系中LOC100294362的表达水平；MCF7细胞转染过表达或干扰LOC100294362后，采用MTT法和Transwell小室法检测上调或沉默LOC100294362对MCF-7细胞增殖和侵袭能力的影响，Western blot法检测p53蛋白表达。结果 与正常组织及细胞相比，LOC100294362在乳腺癌组织和细胞中显著高表达；过表达LOC100294362导致MCF-7细胞增殖和侵袭能力增加，p53蛋白表达降低；沉默LOC100294362导致MCF-7细胞增殖和侵袭能力受到抑制，p53蛋白表达升高。结论 LOC100294362可能通过抑制p53蛋白的表达从而促进乳腺癌细胞的增殖和侵袭
Full-Text
