%0 Journal Article
%T 长链非编码RNA LOC100294362对乳腺癌细胞增殖和侵袭的影响
%A 何剑琴
%A 凌宏艳
%A 奉水东
%A 张恺芳
%A 朱责梅
%A 杨丝丝
%A 阳际花
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.05.010
%X 摘要 目的 探讨长链非编码RNA LOC100294362在乳腺癌组织及细胞系中的表达情况，观察LOC100294362对乳腺癌MCF-7细胞增殖和侵袭的影响，并探索其可能的作用机制。方法 采用定量反转录PCR（qRT-PCR）方法检测乳腺癌组织及细胞系中LOC100294362的表达水平；MCF7细胞转染过表达或干扰LOC100294362后，采用MTT法和Transwell小室法检测上调或沉默LOC100294362对MCF-7细胞增殖和侵袭能力的影响，Western blot法检测p53蛋白表达。结果 与正常组织及细胞相比，LOC100294362在乳腺癌组织和细胞中显著高表达；过表达LOC100294362导致MCF-7细胞增殖和侵袭能力增加，p53蛋白表达降低；沉默LOC100294362导致MCF-7细胞增殖和侵袭能力受到抑制，p53蛋白表达升高。结论 LOC100294362可能通过抑制p53蛋白的表达从而促进乳腺癌细胞的增殖和侵袭
%K 长链非编码RNA
%K LOC100294362
%K MCF-7细胞
%K 增殖
%K 侵袭
%K p53
%K Autophagic Cell Death Induced by GANT61 Inhibits Proliferation of Human Breast Cancer Cell Line MCF-7
%K Effects of RNAi Silencing GTPBP4 Gene on Proliferation and Apoptosis of Colon Cancer RKO Cells
%K Effect of Thyroid Hormone Receptor βΔ on Proliferation and Apoptosis of Rat Breast Cancer Cells SHZ-88
%K Effect of Down-regulating KLF8 Expression by siRNA on EMT in Nasopharyngeal Carcinoma Cells
%K Mechanism of Ibuprofen Regulating Migration and Invasion of Liver Cancer QGY-7703 Cells Through PI3K/Akt/mTOR Signaling Pathway
%K Effects of GSTP1 on Proliferation and Invasion of Human HepG2 Cells
%K Effect of SAHA on Proliferation and Apoptosis of Breast Cancer Cells MDA-MB-435 and Related Mechanism
%K Effect of PTTG1 Expression on Invasion and Migration of Colon Cancer Cell SW480 and Its Possible Mechanism
%K Effect of RORα Overexpression on Proliferation
%K Migration and Invasion of Human Gastric MGC803 Cells
%K Effects of FEN1 Overexpression on Biological Behaviors of Hepatocellular Carcinoma Cells and Prognosis of Patients
%K Effect of Ectopic Expression of Transmembrane Adaptor PAG1 on Motility of Cutaneous Squamous Cell Carcinoma A431 Cells in vitro
%K Effects on Cell Proliferation
%K Invasion and Migration of Esophageal Adenocarcinoma by Inhibition of Notch and PI3K /Akt Pathway
%K Effect of Rap2a on Invasion and Metastasis of Lung Cancer Cells and Its Related Mechanism
%K Progress of TBX3 in Development of Malignant Tumors
%K Effect of Vascular Endothelial Cadherin on Invasion and Metastasis of Non-small Cell Lung Cancer Cells and Related Mechanism
%U http://www.zlfzyj.com/CN/abstract/abstract8748.shtml