NF-κB与放射线诱导的人食管癌细胞上皮间质转化间的关系
DOI: 10.3971/j.issn.1000-8578.2016.11.001
Keywords: 食管癌,放射抵抗性,放射治疗,NF-&kappa,B,E-钙黏蛋白,波形蛋白,CircHIPK3 Affects Malignant Phenotypes in Esophageal Squamous Cell Carcinoma by Regulating p53-Akt-Mdm2 Signaling Pathways,Antitumor Efficacy and Mechanisms of Lapatinib in Combination with Cisplatin on Esophageal Squamous Cancer Cells,Expression of Infiltrating NK Cell Surface Receptors in Peripheral Blood and Cancer Tissues of Patients with Esophageal Squamous Cell Carcinoma,Risk Factors of Pathological Upstaging for Patients with Clinical T2N0M0 Esophageal Squamous Cell Carcinoma,Comparison of Survival Status and Related Risk Factors of Esophageal Squamous Cell Carcinoma Patients Between High- and Low-incidence Areas of ESCC,Expression of Programmed Cell Death 1 and Lymphocyte Activation Gene 3 in Perioperation of Adenocarcinoma of Esophagogastric Junction and Related Significance,Effect of Selective Increase of Single Dose of PTV on Prognosis of Patients with Esophageal Cancer,Silence of STAT3 Enhances SNX-2112-induced Apoptosis of Esophageal Cancer Stem Cells,Microarray Analysis of Radioresistance-associated Genes in Esophageal Squamous Cell Carcinoma,Down-regulation of VRK1 Expression by siRNA Suppresses BANF1 Expression and Proliferation and Migration Abilities of Esophageal Squamous Cell Carcinoma Cells,Effect and Mechanism of Metformin on Side Population Cells of Human Esophageal Squamous Cell Carcinoma,Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues,Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma,Application of COMPASS 3D Dose Validation System in Volumetric Modulated Arc Therapy of Esophageal Cancer,Research Progress of Long Non-coding RNA in Esophageal Cancer
Abstract:
摘要 目的 探讨NF-κB与放射线诱导的人食管癌细胞上皮间质转化（EMT）间的关系。方法 累积照射食管癌Eca109细胞60 Gy，得到Eca109R60细胞，克隆形成实验检测其放射抵抗性，显微镜下观察细胞形态学变化。Real-time PCR和免疫细胞化学检测Eca109和Eca109R60细胞NF-κB、E-cadherin和Vimentin的表达情况。结果 Eca109R60细胞具备了EMT样表型且放射抵抗性更强。Eca109R60细胞中E-cadherin mRNA表达明显下调（P=0.003），Vimentin、NF-κB mRNA表达升高（P=0.004，P=0.004）。相关性分析显示NF-κB与E-cadherin mRNA表达呈负相关，与Vimentin未见有明显相关性，但已显示出正相关的趋势。免疫细胞化学法检测Eca109R60细胞E-cadherin阳性染色程度明显减弱，Vimentin、NF-κB p65阳性染色程度明显增强。结论 Eca109R60细胞放射抗性可能由EMT与NF-κB信号通路相互作用调控
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