%0 Journal Article
%T NF-κB与放射线诱导的人食管癌细胞上皮间质转化间的关系
%A 冉玉格
%A 刘军领
%A 周欢娣
%A 张歌
%A 杨晔
%A 肖志清
%A 薛晓英
%J 肿瘤防治研究
%D 2016
%R 10.3971/j.issn.1000-8578.2016.11.001
%X 摘要 目的 探讨NF-κB与放射线诱导的人食管癌细胞上皮间质转化（EMT）间的关系。方法 累积照射食管癌Eca109细胞60 Gy，得到Eca109R60细胞，克隆形成实验检测其放射抵抗性，显微镜下观察细胞形态学变化。Real-time PCR和免疫细胞化学检测Eca109和Eca109R60细胞NF-κB、E-cadherin和Vimentin的表达情况。结果 Eca109R60细胞具备了EMT样表型且放射抵抗性更强。Eca109R60细胞中E-cadherin mRNA表达明显下调（P=0.003），Vimentin、NF-κB mRNA表达升高（P=0.004，P=0.004）。相关性分析显示NF-κB与E-cadherin mRNA表达呈负相关，与Vimentin未见有明显相关性，但已显示出正相关的趋势。免疫细胞化学法检测Eca109R60细胞E-cadherin阳性染色程度明显减弱，Vimentin、NF-κB p65阳性染色程度明显增强。结论 Eca109R60细胞放射抗性可能由EMT与NF-κB信号通路相互作用调控
%K 食管癌
%K 放射抵抗性
%K 放射治疗
%K NF-&kappa
%K B
%K E-钙黏蛋白
%K 波形蛋白
%K CircHIPK3 Affects Malignant Phenotypes in Esophageal Squamous Cell Carcinoma by Regulating p53-Akt-Mdm2 Signaling Pathways
%K Antitumor Efficacy and Mechanisms of Lapatinib in Combination with Cisplatin on Esophageal Squamous Cancer Cells
%K Expression of Infiltrating NK Cell Surface Receptors in Peripheral Blood and Cancer Tissues of Patients with Esophageal Squamous Cell Carcinoma
%K Risk Factors of Pathological Upstaging for Patients with Clinical T2N0M0 Esophageal Squamous Cell Carcinoma
%K Comparison of Survival Status and Related Risk Factors of Esophageal Squamous Cell Carcinoma Patients Between High- and Low-incidence Areas of ESCC
%K Expression of Programmed Cell Death 1 and Lymphocyte Activation Gene 3 in Perioperation of Adenocarcinoma of Esophagogastric Junction and Related Significance
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%K Silence of STAT3 Enhances SNX-2112-induced Apoptosis of Esophageal Cancer Stem Cells
%K Microarray Analysis of Radioresistance-associated Genes in Esophageal Squamous Cell Carcinoma
%K Down-regulation of VRK1 Expression by siRNA Suppresses BANF1 Expression and Proliferation and Migration Abilities of Esophageal Squamous Cell Carcinoma Cells
%K Effect and Mechanism of Metformin on Side Population Cells of Human Esophageal Squamous Cell Carcinoma
%K Expression and DNA Methylation Status of Long Non-coding RNA ZNF667-AS1 in Esophageal Squamous Cell Carcinoma Tissues
%K Expression and Clinical Significance of miRNA-181d in Esophageal Squamous Cell Carcinoma
%K Application of COMPASS 3D Dose Validation System in Volumetric Modulated Arc Therapy of Esophageal Cancer
%K Research Progress of Long Non-coding RNA in Esophageal Cancer
%U http://www.zlfzyj.com/CN/abstract/abstract8863.shtml