Light sheet imaging comes of age

Light-sheet fluorescence microscopy methods that minimize photodamage are increasingly penetrant in biomedical research and currently comprise three distinct platforms. In this issue, Fadero et al. (2018. J. Cell Biol. https://doi.org/10.1083/jcb.201710087) describe a fourth approach termed “LITE microscopy,” which is useful for extended imaging of specimens from cells to organisms.