Endometrial regeneration using cell sheet transplantation techniques in rats facilitates successful fertilization and pregnancy - Fertility and Sterility

To regenerate functional endometrium tissue using “cell sheet” techniques as a regenerative medicine approach to address endometrial disorders causing female factor infertility. In vivo experimental study. Preclinical surgical and biomedical research laboratories. Green fluorescent protein (GFP) transgenic rats [SD-Tg (CAG-EGFP) rats] and nude rats (F344/NJcl-rnu/rnu). GFP-positive rat uterine-derived cells as cell sheets were transplanted into resected rat uterine endometrial sites. Transplanted cell sheet areas were then analyzed using macroscopic observations and histological analysis including immunohistochemistry. Subsequently, crossbreeding was performed to establish fertility and confirm pregnancy in the rat-regenerated uterus. Morphologic and biochemical markers of regenerated endometrium and establishment of pregnancy in otherwise sterile animals. After cell sheet transplantation, regenerated endometrium was confirmed as GFP-positive tissue engraftment both visually and under histological analysis. After crossbreeding, GFP-positive tissue areas and living fetuses were observed in the transplantation group. Cell sheet transplantation can regenerate endometrial tissue with histological structure and physiological function supporting pregnancy similar to normal endometrial tissue. Translation of this endometrial cell sheet transplantation method to human patients with endometrial disorders could yield a novel therapy for uterine infertility. La regeneración endometrial utilizando técnicas de trasplante de láminas celulares facilita el éxito de la fertilización y el embarazo en ratas. Regenerar tejido endometrial funcional, utilizando técnicas de “láminas celulares” en el contexto de la medicina regenerativa para tratar trastornos endometriales que causan infertilidad por factor femenino. Estudio experimental in vivo. Laboratorios de investigación preclínica quirúrgica y biomédica. Ratas transgénicas marcadas con proteína fluorescente verde (GFP) [ratas SD-Tg (CAG-EGFP)] y ratas nude (F344 / NJcl-rnu / rnu). Células derivadas de úteros de ratas GFP positivas fueron trasplantadas usando láminas celulares en endometrios uterinos de ratas reseccionadas. Las áreas de láminas celulares trasplantadas se analizaron mediante observaciones macroscópicas y análisis histológicos incluyendo inmunohistoquímica. Posteriormente, se realizaron cruzamientos para establecer la fertilidad y confirmar los embarazos en los úteros regenerados por las ratas. Marcadores morfológicos y bioquímicos de regeneración endometrial y consecución de embarazo en animales, de lo