%0 Journal Article
%T Endometrial regeneration using cell sheet transplantation techniques in rats facilitates successful fertilization and pregnancy - Fertility and Sterility
%A Goro Kuramoto
%A Hideo Matsui
%A Ken Ishitani
%A Soichi Takagi
%A Tatsuya Shimizu
%A Teruo Okano
%J Fertility and Sterility
%D 2018
%R https://doi.org/10.1016/j.fertnstert.2018.03.007
%X To regenerate functional endometrium tissue using ¡°cell sheet¡± techniques as a regenerative medicine approach to address endometrial disorders causing female factor infertility. In vivo experimental study. Preclinical surgical and biomedical research laboratories. Green fluorescent protein (GFP) transgenic rats [SD-Tg (CAG-EGFP) rats] and nude rats (F344/NJcl-rnu/rnu). GFP-positive rat uterine-derived cells as cell sheets were transplanted into resected rat uterine endometrial sites. Transplanted cell sheet areas were then analyzed using macroscopic observations and histological analysis including immunohistochemistry. Subsequently, crossbreeding was performed to establish fertility and confirm pregnancy in the rat-regenerated uterus. Morphologic and biochemical markers of regenerated endometrium and establishment of pregnancy in otherwise sterile animals. After cell sheet transplantation, regenerated endometrium was confirmed as GFP-positive tissue engraftment both visually and under histological analysis. After crossbreeding, GFP-positive tissue areas and living fetuses were observed in the transplantation group. Cell sheet transplantation can regenerate endometrial tissue with histological structure and physiological function supporting pregnancy similar to normal endometrial tissue. Translation of this endometrial cell sheet transplantation method to human patients with endometrial disorders could yield a novel therapy for uterine infertility. La regeneraci¨®n endometrial utilizando t¨¦cnicas de trasplante de l¨¢minas celulares facilita el ¨¦xito de la fertilizaci¨®n y el embarazo en ratas. Regenerar tejido endometrial funcional, utilizando t¨¦cnicas de ¡°l¨¢minas celulares¡± en el contexto de la medicina regenerativa para tratar trastornos endometriales que causan infertilidad por factor femenino. Estudio experimental in vivo. Laboratorios de investigaci¨®n precl¨ªnica quir¨²rgica y biom¨¦dica. Ratas transg¨¦nicas marcadas con prote¨ªna fluorescente verde (GFP) [ratas SD-Tg (CAG-EGFP)] y ratas nude (F344 / NJcl-rnu / rnu). C¨¦lulas derivadas de ¨²teros de ratas GFP positivas fueron trasplantadas usando l¨¢minas celulares en endometrios uterinos de ratas reseccionadas. Las ¨¢reas de l¨¢minas celulares trasplantadas se analizaron mediante observaciones macrosc¨®picas y an¨¢lisis histol¨®gicos incluyendo inmunohistoqu¨ªmica. Posteriormente, se realizaron cruzamientos para establecer la fertilidad y confirmar los embarazos en los ¨²teros regenerados por las ratas. Marcadores morfol¨®gicos y bioqu¨ªmicos de regeneraci¨®n endometrial y consecuci¨®n de embarazo en animales, de lo
%U https://www.fertstert.org/article/S0015-0282(18)30260-7/fulltext