The absolute configuration of mitomycin C was determined？by X-ray single crystal diffraction？(CuKα), and a new？crystalline？dihydrate of mitomycin C had been prepared. The？experiment？result？provides？a definitive answer？to the real absolute configuration of mitomycin C and may put an end to the dispute？that baffles？researchers for decades.？At the same time, some contentious structures？about the mitomycin C in？American Pharmacopoeia？USP36-NF31,？Chinese pharmacopoeia？2015？edtion？and numbers of？literatures are marked. The absolute configuration？is？also verified？by？
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