峨眉拟单性木兰嫩茎诱导及再生培养研究
Study on Shoot Induction and Tissue Culture Regeneration of Parakmeria omeiensi

DOI: 10.12677/BR.2020.92016, PP. 132-138

Keywords: 拟单性木兰，嫩茎，再生，植物生长调节剂
Parakmeria omeiensis, Shoot, Regeneration, Plant Growth Hormone

Full-Text   Cite this paper   Add to My Lib

Abstract:

为奠定人工繁育和保护研究的基础，对峨眉拟单性木兰嫩茎诱导培养、不定芽的分化及组培苗再生进行了研究，建立起培养周期短、再生效率高的峨眉拟单性木兰组织培养体系。利用多因子正交实验设计及分析，筛选出最佳诱导培养基是MS + 2.0 mg/L 6-BA + 1.5 mg/L KT + 0.02 mg/L IAA。通过比较植物生长调节剂，发现最适的分化培养基为MS + 0.5 mg/L 6-BA + 0.02 mg/L NAA。最后，生根培养基以MS培养基添加NAA 0.5 mg/L，IBA 2.5 mg/L能有效地使再生苗产生根系。
Through the study of shoot induction culture, adventitious bud differentiation and plantlet rege-neration, a tissue culture system was established, which was short culture cycle and high regene-ration efficiency. The design and analysis of multivariate experiments showed, the best induction medium was MS + 2.0 mg/L 6-BA + 1.5 mg/L KT + 0.02 mg/L IAA. By comparing plant growth hor-mone, we found that the most suitable medium for differentiation was MS + 0.5 mg/L 6-BA + 0.02 mg/L NAA. Finally, MS medium with NAA 0.5 mg/L and IBA 2.5 mg/L can effectively produce roots of regenerated seedlings.

References

[1]	胡先骕, 郑万钧, 拟克枺丽木. 中国西南部木兰科一新属[J]. 中国科学院大学学报, 1951, 1(1): 1-3.
[2]	刘玉壶. 木兰科分类系统的初步研究[J]. 植物分类学报, 1984, 22(2): 88-107.
[3]	刘玉壶, 夏念和, 杨惠秋. 木兰科的起源、进化和地理分布[J]. 热带亚热带植物学报, 1995, 3(4): 1-12.
[4]	徐凤霞, 吴七根. 木兰科种子内种皮合点区形态及其系统学意义[J]. 植物分类学报, 2002, 40(3): 260-270.
[5]	林祁, 段林东, 袁琼, 李明红, 谢永红. 拟单性木兰属(木兰科)植物的分类学修订[J]. 植物研究, 2006, 26(5): 527-531.
[6]	中国环境与发展国际合作委员会生物多样性工作组. 中国物种红色名录(第一卷) [M]. .北京: 高等教育出版社, 2004.
[7]	庄平, 刘仁英, 梁开和. 峨眉拟单性木兰群落特征的初步研究[J]. 广西植物, 1993(1): 61-69.
[8]	Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-479. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[9]	马玲, 张鑫, 孟莹, 赵静, 张满让. 喷施GA3和6-BA对“富士”苹果顶芽内源激素及成花成枝的影响[J]. 西北植物学报, 2018, 38(5): 873-884.
[10]	Gu, X.F. and Zhang, J.R. (2005) An Efficient Adventitious Shoot Regeneration System for Zhanhua Winter Jujube (Zizyphus jujuba Mill.) Using Leaf Explants. Plant Cell Reports, 23, 775-779. https://doi.org/10.1007/s00299-005-0920-5
[11]	Zheng, X.B. (2003). Studies on Callus Induction and Plantlet Regeneration of Triploid Citrullus lanatus Seedings. Henan Agricultural University, Zhengzhou.
[12]	Mithila, J., Hall, J.C., Victor, J.M.R. and Saxena, P.K. (2003) Thidiazuron Induces Shoot Organogenesis at Low Concentrations and Somatic Embryogenesis at High Concentrations on Leaf and Petiole Explants of African Violet (Saintpaulia ionantha Wendl.). Plant Cell Reports, 21, 408-414. https://doi.org/10.1007/s00299-002-0544-y

Full-Text